不同去势时间大鼠牙槽骨微结构变化的Micro-CT研究

发布时间：2019-06-22 10:39

【摘要】：目的 :观察不同去势时间大鼠牙槽骨微结构的改变,探讨去势时间对牙槽骨骨质疏松程度的影响。方法 :健康未孕雌性SD大鼠24只,随机分为去势组(OVX)与假手术组(sham),每组各12只,分别在全麻下行双侧卵巢去势术与假手术。于术后3个月及6个月2个不同时间点分别处死每组大鼠各6只,取右侧上颌骨标本行Micro-CT扫描;观察去势3个月及6个月后大鼠上颌第一磨牙根分叉区牙槽骨微结构的改变。采用SPSS16.0软件包对数据进行统计学分析。结果:Micro-CT水平面、矢状面二维图像及三维重建均显示,去势3个月及6个月大鼠牙槽骨骨髓腔面积增加、骨小梁变细、部分发生断裂。骨结构参数分析显示,去势3个月及6个月后,大鼠牙槽骨骨密度(BMD)及骨体积分数(BV/TV)、骨小梁宽度(Tb.Th)均显著低于相应时间假手术大鼠(P0.05);去势6个月组较3个月组大鼠牙槽骨骨密度(BMD)、骨体积分数(BV/TV)、骨小梁宽度(Tb.Th)显著降低(P0.05)。去势3个月及6个月大鼠骨小梁数目(Tb.N)及骨小梁分离度(Tb.Sp)显著高于相应假手术组(P0.05),且去势6个月组骨小梁数目(Tb.N)显著高于去势3个月组(P0.05)。结论:随着去势时间延长,大鼠牙槽骨骨量降低、骨小梁微结构破坏加剧,骨质疏松程度加重。
[Abstract]:Objective: to observe the changes of alveolar bone microstructure in rats with different ovariectomy time and to explore the effect of ovariectomy time on the degree of alveolar osteoporosis. Methods: 24 healthy unpregnant female SD rats were randomly divided into castrated group (n = 12) and pseudo-operation group (n = 12). Bilateral ovariectomy and pseudo-operation were performed under general anesthesia. Six rats in each group were killed at 3 months and 6 months after operation, and the right maxillary specimens were taken for Micro-CT scanning, and the changes of alveolar bone microstructure in the root bifurcation area of maxillary first molar were observed 3 months and 6 months after castration. SPSS16.0 software package was used to analyze the data statistically. Results: two-dimensional images and three-dimensional reconstruction of Micro-CT horizontal plane, sagittal plane and three-dimensional reconstruction showed that the area of alveolar bone marrow cavity increased, the bone cerebellum became thinner and part of the alveolar bone broke after 3 and 6 months of castration. Bone structure parameters analysis showed that alveolar bone mineral density (BMD), bone volume fraction (BV/TV) and bone trabecular width (Tb.Th) were significantly lower in ovariectomy group than those in 3-month group (P 0.05), and (BMD), bone mineral density (BV/TV) and bone trabecular width (Tb.Th) in castrated 6-month group were significantly lower than those in 3-month group (P 0.05). The number of bone trabeculae (Tb.N) and bone trabecular separation (Tb.Sp) in castrated rats for 3 months and 6 months were significantly higher than those in the corresponding pseudo-operation group (P 0.05), and the number of bone trabeculae (Tb.N) in the castrated 6-month group was significantly higher than that in the castrated 3-month group (P 0.05). Conclusion: with the prolongation of ovariectomy time, the bone mass of alveolar bone decreases, the microstructure of bone trabeculae intensifies, and the degree of osteoporosis aggravates.
【作者单位】：上海交通大学医学院附属第九人民医院·口腔医学院口腔颅颌面科上海市口腔医学重点实验;
【基金】：国家自然科学基金(81371121,11342005,30901698,10972142) 上海市科学技术委员会资助项目(13ZR1423700,12JC1405700) 上海交通大学医工交叉基金(YG2012MS40) SMC-晨星青年学者奖励计划优秀青年教师(B类计划) 上海市教育委员会创新团队项目~~
【分类号】：R782.1

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