钾离子通道阻滞剂4-氨基吡啶对人舌鳞癌细胞迁移和侵袭的影响

发布时间：2019-07-13 16:20

【摘要】：目的:研究4-氨基吡啶(4-AP)对人舌鳞状细胞癌(OTSCC)Tca8113细胞体外迁徙和侵袭能力的影响。方法:采用划痕愈合方法及Transwell侵袭实验检测10、20、30 mmol/L 4-AP干预Tca8113细胞后对Tca8113细胞迁徙及侵袭能力的影响。结果:浓度分别为10 mmol/L、20 mmol/L、30 mmol/L 4-AP干预Tca8113细胞后,Tca8113细胞12 h迁徙率分别为(14.07±5.59)%、(11.34±1.79)%及(8.71±3.26)%;24 h的迁徙率分别为(22.20±5.30)%、(14.24±2.52)%及(11.65±2.97)%,均明显小于对照组(19.25±4.50)%和(33.16±6.40)%,差异具有显著性(P0.01)。细胞侵袭实验结果显示,10 mmol/L、20 mmol/L、30 mmol/L的4-AP作用于Tca8113细胞24 h后穿膜细胞数分别为(112.20±24.36)、(28.87±6.47)、(11.80±5.77)个,随着4-AP浓度的升高,Tca8113穿膜细胞数明显下降,与对照组(179.73±16.14)相比,差异具有显著性(P0.001)。结论:4-AP具有抑制Tca8113舌鳞癌细胞株体外迁徙侵袭的能力。
[Abstract]:Aim: to study the effect of 4-aminopyridinium (4-AP) on migration and invasion of human tongue squamous cell carcinoma (OTSCC) Tca8113) cells in vitro. Methods: scratch healing method and Transwell invasion test were used to detect the effect of 10, 20 and 30 mmol/L 4-AP intervention on the migration and invasion of Tca8113 cells. Results: after Tca8113 cells were treated with 10 mmol/L,20 mmol/L,30 mmol/L 4-AP at 12 h, the migration rates of Tca8113 cells were (14.07 卤5.59)%, (11.34 卤1.79)% and (8.71 卤3.26)%, respectively. The migration rates of 24 h were (22.20 卤5.30)%, (14.24 卤2.52)% and (11.65 卤2.97)%, respectively, which were significantly lower than those of the control group (19.25 卤4.50)% and (33.16 卤6.40)% (P 0.01). The results of cell invasion test showed that the number of transmembrane cells in Tca8113 cells treated with 10 mmol/L,20 mmol/L,30 mmol/L 4-AP for 24 h was (112.20 卤24.36), (28.87 卤6.47), (11.80 卤5.77). With the increase of 4-AP concentration, the number of Tca8113 transmembrane cells decreased significantly, which was significantly different from that in the control group (179.73 卤16.14). Conclusion: 4-AP has the ability to inhibit the migration and invasion of Tca8113 tongue carcinoma cell line in vitro.
【作者单位】：泸州医学院附属口腔医院口腔内科;泸州医学院口颌面修复和再生实验室;重庆市沙坪坝区人民医院口腔科;
【基金】：泸州市科技局科研项目[2013-S-50(3/3)]
【分类号】：R739.86

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