复发性口腔溃疡患者肠道菌群与粪便炎性因子的相关性（英文）

发布时间：2021-02-04 08:37

　　目的探讨肠道微生物变化与复发性口腔溃疡（ROU）的相关性,进而确定肠道微生物变化是否是导致ROU的致病因素之一。方法选取8名经常发作ROU作为实验组（HR）; 14名健康志愿者作为对照组（HN）。对粪便进行宏基因组测序,同时用ELISA检测粪便中炎症因子白细胞介素（IL）-10和IL-22。结果在门水平上,HR中厚壁菌门的丰度高于HN（P<0.05）。在属水平上,HN中Escherichia/Shigella和双歧杆菌的丰度高于HR（P<0.05）。HR组肠球菌的丰度高于HN组（P<0.05）。粪便中IL-10水平在HN、HR组中相似,两组间差异无统计学意义（P>0.05）。HR组IL-22水平高于HN组,两组间差异有统计学意义（P<0.05）。单因素Logistic回归分析显示IL-22是ROU的危险因素。多因素Logistic回归分析显示,双歧杆菌丰度可能是ROU的保护因素,粪便中IL-22可能是危险因素。结论 ROU患者粪便中IL-22与肠道菌群之间存在相互作用,双歧杆菌属丰度显著降低及IL-22水平的升高参与了ROU的发生,且IL-22在ROU发生... 

【文章来源】：解剖学报. 2020,51(04)北大核心

【文章页数】：10 页

【文章目录】：
Objects and Methods
    1. Experimental object and grouping
        1.1 Inclusion criteria:
        1.2 Exclusion criteria:
    2. Feces sample collection
    3. High-throughput sequencing
        3.1 Sample collection:
        3.2 DNA extraction:
        3.3 Library preparation and Illumina
    4. Taxonomic assignment of bacterial 16S rDNA sequencesand data analysis
    5. ELISA detecting the expression of IL-10 and IL-22 in faeces
    6.Data analysis
Results
    1.Sequencing data evaluation
    2.Alpha-diversity analysis
    3.Intestinal microbial community structura features and core flora between the HR and the HN
    4.Principal component analysis based on OTUs
    5. Uni Fracβdiversity analysis
    6. Functional abundance comparison and difference analysis between the two groups
    7. Expression of IL-10 and IL-22 in faeces
Discussion


【参考文献】：
期刊论文
[1]Interleukin-22 receptor 1 is expressed in multinucleated giant cells:A study on intestinal tuberculosis and Crohn’s disease[J]. Zi-Qi Yu,Wen-Fei Wang,You-Chao Dai,Xin-Chun Chen,Jian-Yong Chen.  World Journal of Gastroenterology. 2019(20)



本文编号：3018044