新疆维、汉族人群MTHFR C677T多态性遗传流行病学调查

发布时间：2018-01-17 02:04

本文关键词：新疆维、汉族人群MTHFR C677T多态性遗传流行病学调查　出处：《新疆医科大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的：研究新疆维、汉族人群5,10-亚甲基四氢叶酸还原酶基因(MTHFR)C677T多态性的分布特点,并预知维、汉族人群对服用甲氨蝶呤后不良反应的差异性。方法：应用限制性片段长度多态性扩增技术(PCR-RFLP)分析MTHFR C677T基因型在新疆134例维族和166例汉族人群的分布频率。结果：新疆汉族人群MTHFR C677T基因型频率分别为C/C21.7%、C/T53.0%和T/T25.3%,维族为C/C16.4%、C/T80.6%和T/T2.96%,二者差异有统计学意义(P0.05)。维族男性该基因型频率分别为16.95%、79.7%和3.39%,女性为9.1%、88.0%和2.67%(P0.05)；汉族男性为25.8%、47.1%和27.1,女性为17.28%、59.26%和23.46%,二者差异无统计学意义(P0.05)。结论：新疆维、汉族MTHFR C677T的三种基因型CC、CT、TT的分布存在差异,三种基因型的分布在性别间未见差异,并预知在临床中如果服用相同剂量甲氨蝶呤时,汉族患者出现不良反应的几率和程度比维族患者要高。同时,服用相同剂量甲氨蝶呤后MTHFR 677TT基因型的患者发生副作用的几率和程度比MTHFR677CC/CT要高。
[Abstract]:Objective: to study the distribution of C677T polymorphism of 5-methylenetetrahydrofolate reductase gene (MTHFRN) in Weiwei, Xinjiang, and to predict the characteristics of the polymorphism. Differences of adverse reactions after methotrexate in Han population methods: PCR-RFLP was used to amplify restriction fragment length polymorphism (RFLP). To analyze the distribution frequency of MTHFR C677T genotypes in 134 Uygur and 166 Han population in Xinjiang. Results: MTHFR in Xinjiang Han population. The frequency of C677T genotypes was C / C 21.7%. C / T 53.0% and T / T 25. 3%, Uygur = C / C 16.4% and T / T 80.6% and T / T 2.96%. The frequency of this genotype in Uygur males was 16.95% and 3.39%, respectively, and that in females was 9.1%. 88.0% and 2.67; 47.1% and 27.1% of Han males and 17.2828% and 23.46% females respectively. Conclusion: the distribution of the three genotypes of MTHFR C677T in Xinjiang and Han nationality is different. The distribution of the three genotypes was not different between sexes, and predicted that if the same dose of methotrexate was taken in clinic, the probability and degree of adverse reactions in Han patients was higher than that in Uygur patients. At the same time. Patients taking the same dose of methotrexate were more likely and more likely to develop side effects than those with MTHFR677CC/CT.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R181.3;R394

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