三氯异氰脲酸和苯扎溴铵对海水养殖源弧菌的抑菌和杀菌效果

发布时间：2018-01-26 21:06

本文关键词： 三氯异氰脲酸苯扎溴铵弧菌最小抑菌浓度杀菌率　出处：《南方水产科学》2015年01期 　论文类型：期刊论文

【摘要】：采用微量二倍稀释法进行三氯异氰脲酸和苯扎溴铵对198株海水养殖源弧菌的体外抑菌试验,在此基础上采用悬液定量杀菌法观察2种消毒剂对2株对虾致病性副溶血弧菌(Vibrio parahaemolyticus)的杀菌效果。结果表明,三氯异氰脲酸对198株弧菌的最小抑菌质量浓度(MIC)为31.13~498.00 mg·L-1,苯扎溴铵对198株弧菌的MIC为20.00~160.00 mg·L-1。在菌悬液杀菌试验中,随着三氯异氰脲酸和苯扎溴铵质量浓度降低,100%杀灭弧菌的作用时间延长。当三氯异氰脲酸质量浓度分别为7.78 mg·L-1和15.56 mg·L-1时,100%杀灭FJ244和FJ246菌株的作用时间均为4 min;当苯扎溴铵质量浓度分别为20.00 mg·L-1和10.00 mg·L-1时,100%杀灭FJ244和FJ246菌株的作用时间分别为3 min和40 min。当消毒剂质量浓度分别递减为上述质量浓度一半时,即使延长消毒剂作用时间也未能完全杀灭弧菌。
[Abstract]:In vitro bacteriostatic test of 198 strains of vibrio from mariculture was carried out by using trace double dilution method with trichloroisocyanuric acid and benzalkonium bromide. The bactericidal efficacy of two disinfectants against two pathogenic Vibrio parahaemolyticus strains of Penaeus penaeus was observed by suspension quantitative germicidal method. The results showed that the germicidal efficacy of the two disinfectants against Vibrio parahaemolyticus was studied. The minimum inhibitory mass concentration (MIC) of trichloroisocyanuric acid on 198 strains of Vibrio vibrio was 31.13498. 00 mg 路L -1. The MIC of benzalkonium bromide against 198 strains of Vibrio vibrio was 20.00 ~ 160.00 mg 路L ~ (-1). In the bactericidal test, the concentration of trichloroisocyanuric acid and benzalkonium bromide decreased with the decrease of the concentration of trichloroisocyanurate and benzalkonium bromide. When the mass concentration of trichloroisocyanuric acid was 7.78 mg 路L ~ (-1) and 15.56 mg 路L ~ (-1), respectively. The time of killing FJ244 and FJ246 strains by 100% was 4 mins. When the concentration of benzalkonium bromide was 20.00 mg 路L -1 and 10.00 mg 路L -1, respectively. The time of killing FJ244 and FJ246 strains by 100% was 3 min and 40 mins respectively, when the disinfectant concentration decreased to half of the above concentration, respectively. Even prolonging the action time of disinfectant can not completely kill Vibrio.
【作者单位】：中国水产科学研究院东海水产研究所农业部东海与远洋渔业资源开发利用重点实验室;上海海洋大学水产与生命学院;
【基金】：中国水产科学研究院基本科研业务费项目(2013A0603) 公益性行业(农业)科研专项经费项目(201203085)
【分类号】：R187
【正文快照】： 为了预防和控制水产养殖动物的传染性疾病,消毒是至关重要的生产环节之一。在水产养殖中常用的消毒剂主要有卤素类、季铵盐类和醛类,主要应用于清塘消毒、苗种消毒、预防和控制养殖过程中的微生物病害等[1-2]。目前水产养殖用消毒剂呈现使用量大、使用频率高、使用浓度低等特

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