一起由腺病毒和肠道病毒混合感染引起的发热呼吸道感染暴发疫情的实验室快速诊断

发布时间：2018-03-03 13:31

本文选题：暴发疫情　切入点：发热呼吸道感染　出处：《现代预防医学》2017年21期 　论文类型：期刊论文

【摘要】：目的对一起婴幼儿不明原因发热呼吸道感染暴发疫情进行实验室快速诊断,探索病因。方法采集患儿咽拭子和痰液标本共19份,对所有标本进行核酸提取、运用荧光PCR和荧光RT-PCR法检测腺病毒等引起急性呼吸道感染的8种18型重要病毒的特异性基因片段。对腺病毒和肠道病毒核酸阳性标本进行序列测定,运用生物学软件对测定序列进行处理分析。结果 19份标本腺病毒(Adenovirus,Ad V)核酸阳性,11份标本肠道病毒(Enterovirus,EV)核酸阳性,4份标本鼻病毒(Rhinovirus,RHV)核酸阳性。Ad V的核酸检出阳性率均为100%,标本采集时间最长为发病后10天。EV在发病3天内痰液标本中核酸检出率为83.33%,发病3天后为42.86%。Ad V的基因型别为Ad V-B7型。结论本起发热呼吸道感染暴发疫情由Ad V-B7型和EV混合感染引起,并且Ad V-B7型感染时间长于EV。
[Abstract]:Objective to infant outbreaks for laboratory diagnosis, explore the causes of infection fever of unknown cause respiratory tract. Methods collecting throat swabs and sputum samples of 19 cases were performed on all specimens, nucleic acid extraction, specific gene fragment using fluorescence PCR and fluorescence RT-PCR method for detection of adenovirus induced acute respiratory tract infection in 8 type 18 An important virus. The nucleic acid of adenovirus and enterovirus positive samples were sequenced, using biological software analysis of sequencing. Results of 19 samples of adenovirus (Adenovirus, Ad V) nucleic acid positive, 11 specimens of enterovirus (Enterovirus, EV) nucleic acid positive, 4 specimens of rhinovirus (Rhinovirus, RHV) positive rate of nucleic acid nucleic acid positive.Ad V was 100%, the longest time samples were collected 10 days after the onset of.EV in the pathogenesis of nucleic acid within 3 days in sputum positive rate was 83.33%, the incidence of 3 days The genotype of 42.86%.Ad V was Ad V-B7 type. Conclusion the outbreak of fever and respiratory tract infection is caused by mixed infection of Ad V-B7 type and EV, and Ad V-B7 type infection is longer than EV..

【作者单位】：江西省疾病预防控制中心;
【分类号】：R181.3;R725.1

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