山东某生猪养殖县农村居民产ESBLs肠杆菌科细菌流行现状及耐药情况分析

发布时间：2018-03-11 00:24

  本文选题：超广谱β-内酰胺酶(ESBLs)　切入点：肠杆菌科细菌　出处：《中国公共卫生》2017年03期 　论文类型：期刊论文

【摘要】：目的了解产超广谱β-内酰胺酶(ESBLs)肠杆菌科细菌在山东某生猪养殖县农村居民中的流行现状及耐药情况,为控制抗生素耐药提供参考依据。方法于2016年7月采用随机抽样方法在山东省东部某畜牧养殖县抽取4个村160名农村常住居民作为研究对象,使用e Swab采样管采集其粪便样本,接种于ESBLs筛选培养基,质谱鉴定菌株型别,根据2015版美国临床和实验室标准进行ESBLs确证试验和药物敏感性试验。结果山东某生猪养殖县160份农村居民的粪便样本中有132份产ESBLs,携带率为82.5%;132份阳性标本中共包含产ESBLs的大肠埃希菌184株和肺炎克雷伯菌23株,共207株菌株;产ESBLs的大肠埃希菌和肺炎克雷伯菌对头孢噻肟、四环素、复方新诺明、环丙沙星、氟苯尼考、庆大霉素、哌拉西林/他唑巴坦、磷霉素、阿莫西林/克拉维酸、呋喃妥因、阿米卡星、头孢西丁、美罗培南、替加环素的耐药率分别为100.0%和100.0%、87.0%和95.7%、82.1%和100.0%、64.7%和30.4%、56.5%和43.5%、53.8%和60.9%、31.0%和78.3%、29.9%和69.6%、20.1%和52.2%、13.0%和30.4%、7.1%和0、4.9%和0、0和0、0和56.5%;产ESBLs的大肠埃希菌和肺炎克雷伯菌中,耐1~2、3~5、6~8和≥9种药物的比例分别为7.3%、50.7%、39.6%和2.4%。结论产ESBLs肠杆菌科细菌在山东农村生猪养殖地区的携带率较高,对头孢噻肟、四环素、复方新诺明等药物耐药性较强。
[Abstract]:Objective to investigate the prevalence and drug resistance of extended-spectrum 尾 -lactamases (ESBLs) bacteria in rural residents of a live pig breeding county in Shandong province. Methods in July 2016, a random sampling method was used to sample 160 rural residents from 4 villages in a livestock farming county in eastern Shandong Province. The fecal samples were collected by e Swab sampling tube. Strain types were identified by mass spectrometry after inoculation in ESBLs screening medium. ESBLs confirmatory test and drug sensitivity test were carried out according to the 2015 US clinical and laboratory standards. Results 132 feces samples from 160 rural residents in a live pig breeding county in Shandong province produced ESBLs.The carrying rate was 82.5% and 132 positive samples. #number0# strains of Escherichia coli and 23 strains of Klebsiella pneumoniae were found to produce ESBLs. ESBLs producing Escherichia coli and Klebsiella pneumoniae against cefotaxime, tetracycline, compound sulfamide, ciprofloxacin, florfenicol, gentamicin, piperacillin / tazobactam, fosfomycin, amoxicillin / clavulanic acid, The drug resistance rates of nitrofurantoin, amikacin, cefoxitin, meropenem and tegacycline were 100.0% and 100.00.087.0% and 95.77.0%, 82.1% and 100.064.7% and 30.4% and 56.5% and 43.53.5% and 43.53.5% and 43.53.5% and 60.931% and 78.329.9% and 69.620.1% and 59.620.1% and 30.47.1% and 30.47.1% and 04.9% and 0, 00, 0 and 56.5of ESBLs, respectively, of Escherichia coli and Klebsiella pneumoniae of ESBLs, The rates of resistance to 1, 2, 2, 3, 3, 5, 5, 5, 5, 5, 8, and 鈮，

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