过氧化氢汽体对细菌芽孢与细菌繁殖体杀灭效果的比较

发布时间：2018-06-18 09:42

本文选题：过氧化氢汽体 + 细菌芽孢　；参考：《中国消毒学杂志》2014年08期

【摘要】：目的比较细菌芽孢与细菌繁殖体对过氧化氢汽体(VPHP)的抗力,为选择VPHP生物指示剂提供参考。方法采用载体定量杀菌实验方法,比较某过氧化氢汽体发生器发生的VPHP对两组常用消毒试验指标菌芽孢与金黄色葡萄球菌杀灭效果,以评价此两种细菌芽孢作为VPHP生物指示剂的可能性。结果该VPHP发生器在20 m3空间内运行0.5~1.0 h,室内中心点过氧化氢浓度均值为1 972~2 615 mg/m3;运行1.5~2.5 h,室内中心点过氧化氢浓度均值为2 474~1 070 mg/m3。启动该VPHP发生器运行2.5 h(一周期),对不锈钢载体上的金黄色葡萄球菌、枯草杆菌黑色变种芽孢、嗜热脂肪杆菌芽孢杀灭对数值分别为2.32、4.18、5.00。结论该VPHP发生器在其设定的运行周期内,对金黄色葡萄球菌的杀灭效果明显低于两种细菌芽孢,提示仅以此两种细菌芽孢作为VPHP生物指示剂存在局限性。
[Abstract]:Objective to compare the resistance of bacterial spores to hydrogen peroxide vapor (VPHP) and to provide reference for the selection of VPHP biological indicator. Methods the carrier quantitative germicidal test method was used to compare the efficacy of VPHP produced by a hydrogen peroxide vapor generator in killing two groups of commonly used disinfection test indexes: spores and Staphylococcus aureus. The possibility of using these two bacterial spores as biological indicators of VPHP was evaluated. Results the average concentration of hydrogen peroxide at the center point of the VPHP generator was 1 972U 2 615 mg / m 3 for 0. 5 h in a space of 20 m ~ 3 and 2. 5 h for 2. 5 h, and the average hydrogen peroxide concentration at the center point of the room was 2 774% 1 070 mg 路m ~ 3 路m ~ (3). Starting the VPHP generator for 2.5 h (one cycle), the logarithmic values of killing Staphylococcus aureus on stainless steel carrier, Bacillus subtilis var. Niger spores, and adipose thermophilic bacillus spores were 2.32 ~ 4.185.00, respectively. Conclusion the efficacy of the VPHP generator in killing Staphylococcus aureus is lower than that of the two bacterial spores during its set operating cycle, suggesting that only these two bacterial spores can be used as the biological indicator of VPHP.
【作者单位】：浙江省疾病预防控制中心;
【基金】：浙江省医药卫生骨干人才类科研基金(2011RCB014)
【分类号】：R187

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