2009年-2013年赣州市禽类养殖人群血清学和环境高致病性禽流感监测分析

发布时间：2019-06-07 16:31

【摘要】：目的监测赣州市禽类养殖人群和环境高致病性禽流感病毒的感染情况,为其预防控制策略的制定提供科学依据。方法根据《全国职业暴露人群血清学和环境高致病性禽流感监测方案》的要求,采用单放射免疫扩散溶血试验(SRH)或马红细胞血凝抑制试验(HI)检测禽类养殖人群H5N1血凝素抗体;采用Real-time PCR对环境标本进行A型/和H5、H7、H9亚型禽流感病毒核酸检测。结果 2009年-2013年5个监测年度共657份禽类养殖人群血清H5N1血凝素抗体均为阴性;621份环境标本禽流感病毒核酸监测A型阳性率为15.78%(其中H5阳性率5.96%,H7阳性率0.97%,H9阳性率0.97%,A未分型8.86%,且H7和H9是混合感染)。结论赣州市禽类养殖监测点环境中存在H5、H7、H9禽流感病毒,存在人感染高致病性禽流感的风险,应加强禽类养殖人群的禽流感病毒感染监测。赣州市尚无人感染H5N1禽流感。
[Abstract]:Objective to monitor the infection of highly pathogenic avian influenza virus in poultry breeding population and environment in Ganzhou City, and to provide scientific basis for its prevention and control strategy. Methods according to the requirements of the National Surveillance Program for Serological and Environmental highly pathogenic Avian Influenza in Occupational exposed population, H5N1 hemagglutinin antibody was detected by single radioimmunodiffusion hemolysis test (SRH) or horse red blood cell hemagglutination inhibition test (HI). Type A / H5, H7, H9 subtypes of avian influenza virus nucleic acid were detected by Real-time PCR. Results from 2009 to 2013, serum H5N1 hemagglutinin antibodies were negative in 657 poultry fowls. The positive rate of avian influenza virus type A in 621 environmental specimens was 15.78% (of which H5 was 5.96%, H7 was 0.97%, H9 was 0.97%, A was 8.86%, and H7 and H9 were mixed infection). Conclusion there are H5, H7 and H9 avian influenza viruses in the environment of poultry culture monitoring sites in Ganzhou City, and there is a risk of human infection with highly pathogenic avian influenza. The surveillance of avian influenza virus infection in poultry breeding population should be strengthened. No one in Ganzhou has been infected with H5N1 avian influenza.
【作者单位】：赣州市疾病预防控制中心;
【分类号】：R181.3

【参考文献】