针刺联合中药对糖尿病模型大鼠胰岛的保护作用

发布时间：2018-01-18 09:02

本文关键词：针刺联合中药对糖尿病模型大鼠胰岛的保护作用　出处：《中国老年学杂志》2015年03期 　论文类型：期刊论文

【摘要】：目的探讨针刺联合中药对糖尿病大鼠胰岛的保护作用及机制。方法健康老年SD大鼠50只,雌雄各半。随机分为:空白组;模型组;针刺组;中药组;针刺+中药组(n=10)。除空白组外,其余各组大鼠经腹腔注射链脲佐菌素(STZ)(45 mg/kg,1次),72 h后经测定血糖确认模型复制成功。针刺组取肺俞、三阴交等两组穴位隔天交替针刺8个疗程(6 d为一疗程);中药组给予白芍甘草汤灌胃(10 ml/kg,1次/d)7 w。针刺+中药组在针刺同时联合白芍甘草汤灌胃。各组动物于7 w后麻醉处死,检测血糖、血胰岛素水平及血清一氧化氧(NO)水平,取胰腺利用RT-PCR及免疫组化检测大鼠胰腺组织中诱导型一氧化氮合酶(i NOS)mRNA和蛋白质的表达变化。结果与空白组比较,模型组大鼠血糖水平明显升高,血中胰岛素的水平明显降低、血清一氧化氮(NO)水平明显升高,胰腺i NOS的表达显著增加(P0.05);与模型组比较,针刺+中药组血糖的水平明显降低,血胰岛素水平明显升高、NO水平明显降低、胰腺i NOS的表达显著降低(P0.05);针刺组或中药组大鼠虽然血糖水平降低,血胰岛素水平升高、NO水平降低、胰腺i NOS的表达降低,但与模型组无差异(P0.05)。结论针刺联合中药能有效促进胰岛素分泌,发挥降血糖的功效,其机制可能与调节i NOS的表达从而降低血清中NO水平有关。
[Abstract]:Objective to investigate the protective effect and mechanism of acupuncture combined with traditional Chinese medicine on islet of diabetic rats. Methods 50 healthy old SD rats, half male and half male, were randomly divided into two groups: blank group; Model group; Acupuncture group; Traditional Chinese medicine group; Except the blank group, rats in other groups were injected intraperitoneally with STZ (45 mg 路kg ~ (-1) 路kg ~ (-1) 路kg ~ (-1))). After 72 hours, the blood glucose was determined and the model was successfully duplicated. The acupuncture group took Feshu and Sanyinjiao alternately acupuncture for 8 courses of treatment for 6 days as a course of treatment. The traditional Chinese medicine group was given 10 ml / kg of Radix Paeoniae Glycyrrhizae decoction for 7 ws.Acupuncture group was combined with Radix Paeoniae Paeoniae Glycyrrhizae decoction for 7 weeks. The animals in each group were anesthetized and killed after 7 weeks of anesthesia. The levels of blood glucose, serum insulin and serum nitric oxide (no) were measured. The expression of inducible nitric oxide synthase (iNOS) I NOS)mRNA and protein in rat pancreas was detected by RT-PCR and immunohistochemistry. In the model group, the blood glucose level was significantly increased, the serum insulin level was significantly decreased, the serum nitric oxide (no) level was significantly increased, and the expression of pancreatic I NOS was significantly increased (P 0.05). Compared with the model group, the level of blood glucose, the level of serum insulin, the level of no and the expression of I NOS in pancreas were significantly decreased in the acupuncture Chinese medicine group. Although blood glucose level decreased, serum insulin level increased and no level decreased, and pancreatic I NOS expression decreased in acupuncture group or traditional Chinese medicine group. Conclusion Acupuncture combined with traditional Chinese medicine can effectively promote insulin secretion and play the role of hypoglycemia. The mechanism may be related to regulating the expression of I NOS and decreasing the level of no in serum.
【作者单位】：陕西中医学院医学技术系免疫及检验教研室;
【基金】：陕西省教育厅自然科学研究项目资助(No.07k225)
【分类号】：R285.5
【正文快照】： 糖尿病表现为血糖升高,胰岛素的水平降低,其发病机制可能和胰岛β细胞的凋亡相关〔1~4〕。糖尿病在中医称之为消渴,古代医家论述消渴病机时大多以阴虚燥热立论,阴虚为本,燥热为标〔5〕。因此中医治疗糖尿病多采用滋阴清热、生津、滋肾等法。白芍苦酸微寒,养血敛阴,甘草舒筋活

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