1,8-cineol作为流感疫苗佐剂提高流感疫苗免疫效果的实验研究

发布时间：2018-01-18 09:21

本文关键词：1,8-cineol作为流感疫苗佐剂提高流感疫苗免疫效果的实验研究　出处：《中国免疫学杂志》2017年11期 　论文类型：期刊论文

【摘要】：目的:评价1,8-cineol作为流感疫苗佐剂提高流感疫苗免疫效果的作用。方法:将80只BALB/c小鼠分为空白对照组、1,8-cineol组、HA组以及HA+1,8-cineol组,每组20只。空白对照组正常饲养,HA组接种0.2μg HA、HA+1,8-cineol组接种(0.2μg HA+100 mg/kg 1,8-cineol)、1,8-cineol组接种(100 mg/kg 1,8-cineol)。除空白组外,每组小鼠分别在第0、7、14天滴鼻免疫。第21天乙醚轻度麻醉小鼠,以10LD50流感病毒FM1感染每组10只小鼠。连续观察6 d后,每组处死10只小鼠,收集血清,采用酶联免疫法(ELISA)检测血清中IgG、IgG1b、IgG2a、IgG2b在450 nm的吸光值(OD值)。摘取肺组织,计算肺指数,检测肺病理。每组剩余10只小鼠感染15LD50FM1,观察15 d,记录感染鼠的死亡情况,计算感染鼠的生存率。结果:与HA组相比,HA+1,8-cineol组能降低感染鼠的肺指数,缓解感染鼠的肺部病变,能够提高血清中IgG、IgG1b、IgG2a的抗体水平,提高感染鼠的生存率。结论:1,8-cineol作为疫苗佐剂,能够加强流感疫苗的免疫效果。
[Abstract]:Objective: to evaluate the effect of 8-cineol as adjuvant of influenza vaccine on the immune effect of influenza vaccine. Methods: 80 BALB/c mice were divided into control group (control group) and control group (n = 80). In 8-cineol group, HA group (n = 20) and HA1-8-cineol group (n = 20) were inoculated with 0.2 渭 g HAH1 in normal control group. The 8-cineol group was inoculated with 0. 2 渭 g HA 100 mg/kg 1 and 8 cineolol group inoculated with 100 mg/kg 1. Except the blank group, mice in each group were given nasal immunization on day 0, 7 and 14, respectively, and on day 21, mice were anesthetized slightly by ether. 10 mice in each group were infected with 10LD50 influenza virus FM1. After 6 days of continuous observation, 10 mice were killed in each group and serum was collected. The absorptivity of IgG1b in serum at 450 nm and the OD value of IgG2b at 450 nm were detected by Elisa. The lung tissue was excised and the lung index was calculated. Lung pathology. The remaining 10 mice in each group were infected with 15LD50 FM1. The death rate of infected mice was recorded and the survival rate of infected mice was calculated. Results: compared with HA group, the survival rate of infected mice was calculated. In 8-cineol group, the lung index of infected rats was decreased, the pulmonary lesion of infected rats was alleviated, and the level of IgG1bG2a antibody in serum was increased. Conclusion the vaccine adjuvant of 1: 1 8-cineol can enhance the immune effect of influenza vaccine.
【作者单位】：广州中医药大学;
【基金】：国家自然科学基金项目(81072948,81470186) 广东省自然科学基金研究团队项目(S2012003000698)
【分类号】：R392
【正文快照】： 流感是常见的呼吸道传染病,可引起较高的死亡率,尤其是新型毒株的出现,可引起世界范围内的流感大流行[1,2]。接种流感疫苗是预防流感感染和阻止大流行的主要手段[3]。临床中主要的流感疫苗有亚单位疫苗、灭活疫苗以及重组疫苗[4]。这些流感疫苗对于同一亚型的流感病毒能够起到

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