黄芪注射液对野百合碱诱导肺动脉高压大鼠的药效学研究

发布时间：2018-03-22 10:44

本文选题：黄芪注射液　切入点：野百合碱　出处：《中药材》2015年04期 　论文类型：期刊论文

【摘要】：目的:研究黄芪注射液对野百合碱诱导肺动脉高压大鼠的治疗作用。方法:将SPF级雄性Wistar大鼠60只,按体质量随机分为5组,即1正常对照组:腹腔注射生理盐水1次;2模型组:腹腔注射野百合碱60 mg/kg,第2天开始腹腔注射等体积生理盐水,1次/d,连续3 w;3黄芪注射液低剂量组:腹腔注射野百合碱60 mg/kg,第2天开始腹腔注射黄芪注射液3 m L/kg;4黄芪注射液高剂量组:腹腔注射野百合碱60 mg/kg,第2天开始腹腔注射黄芪注射液6 m L/kg;5卡托普利阳性对照组:腹腔注射野百合碱60 mg/kg,第2天开始给予卡托普利7 mg/kg灌胃,1次/d,连续给药3 w。末次给药1 h后,麻醉动物,采用右心导管法测定大鼠肺动脉压。测压后在腹腔静脉抽血检测血浆ET-1、TXB2及NO含量。取心脏,分离并称取右心室(RV)和左心室加室间隔(LV+S)的质量,并以RV/(LV+S)比值作为右心肥厚指数。取肺组织做HE切片,观察病理改变并计算肺小动脉中膜厚度百分比。结果:黄芪注射液可降低野百合碱致肺动脉高压大鼠的平均肺动脉压、右心室肥厚指数、肺小动脉中膜厚度及血浆ET-1、TXB2含量,改善肺组织病理学改变。结论:黄芪注射液对野百合碱诱导的大鼠肺动脉高压有一定的防治作用。
[Abstract]:Objective: To study the therapeutic effect of Astragalus Injection on pulmonary hypertension induced by monocrotaline in rats. Methods: Wistar male SPF 60 rats were randomly divided into 5 groups: 1 normal control group: intraperitoneal injection of saline 1 times; 2 model group: intraperitoneal injection of monocrotaline 60 mg/kg second day, intraperitoneal injection of normal saline 1 /d for 3 W; 3 low dose astragalus injection group: intraperitoneal injection of monocrotaline 60 mg/kg, second day of intraperitoneal injection of Huangqi injection 3 m L/kg; 4 high dose astragalus injection group: intraperitoneal injection of monocrotaline 60 mg/kg, second day intraperitoneal injection of Astragalus Injection 6 m L/kg; 5 Kato Pury positive control group: intraperitoneal injection of monocrotaline 60 mg/kg, second days to give Kato Pury 7 mg/kg orally, 1 times /d, continuous administration of 3 W. 1 h after the last administration, animal anesthesia, by right heart catheterization method for the determination of pulmonary arterial pressure in rats. Manometry After the abdominal vein blood plasma ET-1, TXB2 and NO content. The heart was taken and separated and weighed the right ventricle (RV) and left ventricle plus septum (LV+S) quality, and RV/ (LV+S) as the ratio of the right ventricular hypertrophy index. Lung tissue HE staining to observe the pathological changes of lung were calculated small percentage of media thickness. Results: the mean pulmonary artery Huangqi injection could decrease monocrotaline induced pulmonary hypertension in rats with pressure, right ventricular hypertrophy index, pulmonary artery intima-media thickness and plasma ET-1, TXB2 content, improve the pathological changes of lung tissues. Conclusion: Astragalus Injection on monocrotaline induced pulmonary artery hypertension in rats has a protective effect.

【作者单位】：中山大学药学院;哈尔滨珍宝制药有限公司;
【分类号】：R285.5

【参考文献】