外周血T细胞及其亚群与末端回肠炎关系的研究

发布时间：2018-05-08 07:14

本文选题：外周血 + T细胞及其亚群　；参考：《南华大学》2016年硕士论文

【摘要】：目的:探讨外周血T淋巴细胞及其亚群构成比例在末端回肠炎发病过程中的变化及其意义。方法:取SD大鼠90只。体重均为200至350g。将大鼠随机分为M、S、C三组。M组:模型组(Model);S组:缝线组(Suture);C组:对照组(Control)。每组30只。三组分别采取不同的处理。M组:回肠远端与结肠近端吻合,制造结肠-回肠反流模型。S组:制造与M组在回肠和结肠相同位置的同等程度的肠道损伤,而不建立反流模型,形成对照。C组:仅麻醉,不行手术。三组大鼠饲养及护理均相同。分别于术后第2、4、8周,每组随机抽取10只大鼠进行静脉采血及取回肠组织。静脉血用流式细胞术检测T细胞亚群。回肠组织制成切片进行病理评分。结果:1.外周血T细胞亚群构成比例检测:(1)实验大鼠DNT细胞构成比例情况:术后M组、S组大鼠DNT细胞构成比例均明显增加,M组更明显(P0.05),到第2周时达到高峰,说明DNT可能参与了急性炎症反应过程。从第2周开始至第八8周数值逐渐下降,S组在第4周时基本恢复正常值,M组较S组慢,M组与S组比较有显著差异(P0.05)。说明DNT细胞在实验末端回肠炎急性期(0-4周)变化最明显。(2)实验大鼠T4细胞构成比例情况:术后M组、S组大鼠T4细胞构成比均明显降低,m组表现更明显(p0.05),术后第2周数值最低值。术后第2周开始,s组大鼠t4细胞构成比开始增加,到第4周时接近正常水平,而同一时期m组维持在低值水平,有少数数值甚至比第2周时更低,4周以后开始回升到正常值水平。说明末端回肠炎的早期(0-4周),t4细胞可能扮演了重要的角色。(3)实验大鼠t8细胞构成比例情况:术后2周内,m组、s组大鼠t8细胞构成比例均增加,m组、s组有显著差异(p0.05)。第4周时,m组t8细胞构成比例进一步升高,而s组基本达到正常水平(p0.05)。第8周时,m组恢复正常水平。说明末端回肠炎发病的4-8周内,t8细胞可能起了着重要的作用。(4)实验大鼠dpt细胞构成比例情况:术后m组大鼠dpt细胞构成比例明显增加,第4周达到高峰,4-8周逐渐下降,到第8周时仍高于c组,而s组dpt细胞数量变化始终不明显,m、s组有显著差异(p0.05)。结果提示,dpt细胞可能参与了末端回肠炎的发病整个过程(0-8周)。2.外周血t细胞亚群构成比例与回肠组织炎症程度的相关性:通过对m组术后病变明显处组织的肉眼和镜下观察发现,在实验性末端回肠的整个发病过程中,随着时间延长,病变处肠组织病理评分越高,炎症表现越严重。通过m组和c组各t细胞亚群构成比与炎症评分的相关性分析表明,dnt、t8、dpt三类细胞与末端回肠组织炎症严重程度具有正相关性(r值分别为0.518、0.482、0.714,p0.05),t4细胞与末端回肠组织炎症严重程度具有负相关性(r=-0.506,P0.05)。结论:1.在ETI中,DNT、T8、DPT三类T细胞亚群增加,T4细胞减少,外周血T细胞各亚群可能均参与了ETI的发病过程。2.DNT、T8、DPT三类细胞与末端回肠组织炎症严重程度具有正相关性,T4细胞与末端回肠组织炎症严重程度具有负相关性,T细胞各亚群可能作为监测末端回肠炎症程度的重要指标。
[Abstract]:Objective: To investigate the changes and significance of the proportion of T lymphocyte and its subgroup in peripheral blood and its subgroup in the pathogenesis of terminal ileocolitis. Methods: 90 SD rats were randomly divided into M, S, C three groups.M groups: model group (Model); S group: suture group (Suture); C group (Control), 30. Three groups were different respectively. The treatment group.M: the distal ileum and the proximal colon anastomosis, the colonic ileum reflux model.S group: the same degree of intestinal injury in the same position of the ileum and colon in the M group, but not the reflux model, the control group of.C: only anesthesia, no operation. The three groups of rats feeding and nursing were the same. Each group followed 2,4,8 weeks after the operation, respectively. Blood sampling and intestinal tissue were taken in 10 rats. The T cell subgroup was detected by flow cytometry. The ileum tissue was made into slices for pathological score. Results: the proportion of T cell subgroups in peripheral blood was detected in 1. peripheral blood: (1) the proportion of DNT cells in experimental rats: M group after operation, the proportion of DNT cells in group S increased obviously, M The group was more obvious (P0.05) and reached the peak at the second week, indicating that DNT might be involved in the acute inflammatory reaction process. From the beginning of second weeks to eighth 8 weeks, the value of the group S was gradually reduced, and the group M was basically recovered at fourth weeks. The group M was slower than the S group, and there was a significant difference between the group of M and the S group (P0.05). The changes showed that the DNT cell at the acute stage of the experimental terminal colitis (0-4 weeks) was changed. Most obvious. (2) the proportion of T4 cells in experimental rats: the ratio of T4 cells in group M, group S and group M was significantly lower than that in group M (P0.05), and the lowest value was at the beginning of the operation. The constitution ratio of T4 cells in group s increased and reached the normal level at fourth weeks, while the M group maintained at a low level at the same time, and there was a low level in the same period. There was a low level in the same period. A few values were even lower than second weeks and began to rise to normal levels after 4 weeks. It was suggested that T4 cells may play an important role in the early stage of terminal colitis (0-4 weeks). (3) the proportion of T8 cells in experimental rats: the proportion of T8 cells in group M and Group s increased in 2 weeks after operation, and in group M, s group had significant difference (P0.05). Fourth weeks. The proportion of T8 cells in the M group increased further, while the s group basically reached the normal level (P0.05). At eighth weeks, the M group returned to the normal level. It indicated that the T8 cells may play an important role in the pathogenesis of terminal ileocolitis. (4) the proportion of DPT cells in experimental rats: the proportion of DPT cells in the group M rats increased significantly after fourth weeks, reaching fourth weeks. At the peak, the 4-8 week decreased gradually and was still higher than the C group at the eighth week, but the number of DPT cells in group s was not significantly changed, and there was a significant difference in M and s group (P0.05). The results suggest that DPT cells may participate in the whole process of terminal ileocolitis (0-8 weeks) and the correlation between the proportion of T lymphocyte subsets in the peripheral blood of.2. and the degree of inflammation in the ileum tissue: through M In group M and C group, the pathological score of the intestinal tissue was higher and the inflammation was more serious in the whole process of the experimental terminal ileum. The correlation between the constituent ratio of T cell subsets and the inflammation score in the group of M and C groups showed that the DNT, T8, DPT were the cells and the end. There is a positive correlation between the inflammatory severity in the end of the ileum (r value is 0.518,0.482,0.714, P0.05), and there is a negative correlation between the inflammatory severity of the T4 cells and the terminal ileum (r=-0.506, P0.05). Conclusion: 1. in ETI, DNT, T8, DPT three T cell subsets increase, T4 cells decrease, and the subgroups of peripheral blood cells may be involved in the pathogenesis of the disease. Process.2.DNT, T8, DPT three cells are positively correlated with the severity of the inflammation in the terminal ileum. The T4 cells are negatively correlated with the severity of the terminal ileum, and the T cell subgroups may be an important indicator for monitoring the degree of terminal ileocolitis.

【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R574

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