麻醉方法对痉挛型瘫痪大鼠动物模型构建的影响

发布时间：2018-10-17 15:08

【摘要】：目的探讨双侧颈总动脉间断性结扎术建立大鼠痉挛型瘫痪模型时的最适麻醉剂量及方式。方法 Wistar大鼠60只,随机分成6组,每组10只,均行双侧颈总动脉间断性结扎术。A组予10%水合氯醛5 ml/kg皮下注射,B组予10%水合氯醛4 ml/kg皮下注射,C组予10%水合氯醛4 ml/kg腹腔注射,D组予10%水合氯醛3 ml/kg皮下注射,E组予10%水合氯醛3 ml/kg腹腔注射,F组予10%水合氯醛2 ml/kg皮下注射。比较每组麻醉起效时间、维持时间、术中死亡率。结果 A组在麻醉中全部死亡,F组无深度麻醉。B组起效时间(6.5±0.7)min,维持时间(121.4±3.9)min,死亡率0;C组起效时间(5.5±1.1)min,维持时间(122.0±3.6)min,死亡率30%;D组起效时间(9.6±0.8)min,维持时间(106.7±3.7)min,死亡率0;E组起效时间(7.4±1.2)min,维持时间(105.3±3.5)min,死亡率20%。皮下注射总体死亡率0,腹腔注射总体死亡率25%。结论双侧颈总动脉间断性结扎术建立大鼠痉挛型瘫痪模型时,采用10%水合氯醛4 ml/kg皮下注射麻醉,大鼠麻醉死亡率低,起效时间短,维持时间长。
[Abstract]:Objective to study the optimal anesthetic dosage and mode of bilateral common carotid artery ligation in the establishment of spastic paralysis model in rats. Methods 60 Wistar rats were randomly divided into 6 groups with 10 rats in each group. Group A received 10% chloral hydrate 5 ml/kg subcutaneously, group B 10% chloral hydrate 4 ml/kg, group C 10% chloral hydrate 4 ml/kg intraperitoneally, group D 10% chloral hydrate 3 ml/kg. Group E was injected with 10% chloral hydrate for 3 ml/kg and group F was subcutaneously injected with 10% chloral hydrate for 2 ml/kg. The onset time, maintenance time and intraoperative mortality of each group were compared. Results Group A died during anesthesia, Group F had no deep anesthesia, Group B (6.5 卤0.7) min, maintenance time (121.4 卤3. 9) min, death rate, Group C (5.5 卤1. 1) min, maintenance time (122.0 卤3. 6) min, death rate 30 + D group (9. 6 卤0. 8) min, maintenance time (106.7 卤3. 7) min, death rate 0 time. The interval (7.4 卤1. 2) min, maintenance time (105.3 卤3. 5) min, mortality was 20. The total mortality was 0 by subcutaneous injection and 25 by intraperitoneal injection. Conclusion the rat model of spastic paralysis established by bilateral common carotid artery ligation was anesthetized by 10% chloral hydrate for 4 ml/kg. The anesthetic mortality was low, the onset time was short, and the maintenance time was long.
【作者单位】：佳木斯大学康复医学院黑龙江省小儿脑性瘫痪防治疗育中心;
【基金】：黑龙江省研究生创新科研项目(No.LM2014-049) 佳木斯大学校级科技创新团队(No.Cxtd-2013-02)
【分类号】：R614

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