腹腔暴露对大鼠肠黏膜屏障的影响

发布时间：2018-10-17 18:59

【摘要】：目的:观察腹腔暴露对大鼠肠黏膜屏障的影响。方法:将30只大鼠随机分为五组,即对照组、假手术组、腹腔暴露1 h组、腹腔暴露2 h组和腹腔暴露3 h组,每组6只。腹腔暴露组大鼠剖腹后暴露腹腔,但不进行任何手术操作,暴露相应时间后关腹。假手术组大鼠仅作腹壁切口,不打开腹腔。对照组大鼠仅作相应的麻醉。术后24 h,取血检测血清D-乳酸水平,并取回肠组织测定肠道FD4清除率、小肠黏膜紧密连接蛋白ZO-1和Occludin的表达水平,同时观察小肠组织的病理变化。结果:腹腔暴露组较对照组大鼠血清D-乳酸水平有所上升,肠道FD4清除率有所增加,小肠黏膜紧密连接蛋白ZO-1和Occludin的表达水平均有所下降,小肠黏膜病理形态有所改变,并且这种差异随腹腔暴露时间的延长而逐渐显著。Pearson相关性检验分析表明,腹腔暴露组大鼠血清D-乳酸水平和肠道FD4清除率与肠黏膜损伤Chiu's评分均呈显著的正相关。结论:腹腔暴露大鼠肠黏膜屏障功能受损,并随着腹腔暴露时间的延长而逐渐加重。
[Abstract]:Objective: to observe the effect of intraperitoneal exposure on intestinal mucosal barrier in rats. Methods: thirty rats were randomly divided into five groups: control group, sham operation group, intraperitoneal exposure group for 1 h, abdominal cavity exposure group for 2 h and abdominal cavity exposure group for 3 h, with 6 rats in each group. The abdominal cavity of the rats in the abdominal exposure group was exposed after caesarean section, but no operation was performed, and the abdominal cavity was closed after the corresponding exposure. In sham operation group, abdominal wall incision was performed only and abdominal cavity was not opened. Rats in the control group were anesthetized only. At 24 hours after operation, the serum D-lactic acid level was detected, the intestinal FD4 clearance rate and the expression levels of ZO-1 and Occludin in intestinal mucosal tight junction protein were measured in ileal tissues. The pathological changes of intestinal tissues were also observed at the same time. Results: compared with the control group, the serum D-lactic acid level, intestinal FD4 clearance rate, intestinal mucosal tight junction protein (ZO-1) and Occludin expression in the abdominal exposure group were increased, and the intestinal mucosal histopathology was changed. The results of Pearson correlation test showed that the serum D- lactic acid level and intestinal FD4 clearance rate were positively correlated with the Chiu's score of intestinal mucosal injury. Conclusion: intestinal mucosal barrier function was impaired in rats exposed to abdominal cavity, and increased gradually with the prolongation of abdominal exposure time.
【作者单位】：南京大学医学院附属金陵医院(南京军区南京总医院)解放军普通外科研究所;复旦大学附属中山医院普通外科;
【基金】：全军十二五重大新上项目(AWS12J001) 江苏省临床医学科技专项(BL2012006)
【分类号】：R656

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