蒙花散对2型糖尿病伴胰岛素抵抗并发周围神经病变大鼠的影响

发布时间：2019-06-02 19:09

【摘要】：目的:探讨蒙花散对胰岛素抵抗并发周围神经病变糖尿病大鼠周围神经功能的影响。方法:采用链脲佐菌素(STZ)诱导建立SD大鼠糖尿病周围神经病变模型,将模型大鼠随机分为模型组、二甲双胍阳性对照组及蒙花散低、中、高剂量组,另设正常对照组。蒙花散低、中、高剂量组分别灌胃给予蒙花散0.5、1、2 g/kg,二甲双胍组大鼠灌胃给予二甲双胍0.15 g/kg,正常对照组和模型组大鼠给予等体积纯净水,各组大鼠均连续给药4 w。末次给药后禁食12 h,水合氯醛麻醉,腹静脉取血检测血清空腹血糖(FBG)、胰岛素(FINS)、丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平,并用肌电图仪检测运动神经传导速度(MNCV)和感觉神经传导速度(SNCV)。结果:与模型组比较,蒙花散中、高剂量组大鼠FBG、血清FINS水平及IRI显著降低(P0.01),坐骨神经MNCV和SNCV显著升高(P0.01),血清SOD、CAT活性著升高(P0.01),血清MDA、CRP、TNF-α及IL-6含量显著降低(P0.01)。结论:蒙花散可改善糖尿病大鼠的胰岛素抵抗及周围神经的功能,其机制可能与抗氧化及降低炎性因子水平有关。
[Abstract]:Objective: to investigate the effect of Menghua Powder on peripheral nerve function in diabetic rats with insulin resistance complicated with peripheral neuropathy. Methods: the diabetic peripheral neuropathy model of SD rats was established by streptozotocin (STZ). The model rats were randomly divided into model group, metformin positive control group, Menghua powder low, middle and high dose groups, and normal control group. The rats in the low, middle and high dose groups were given Menghua Powder 0.5 g, 1 g 路kg ~ (- 1) and 2 g 路kg ~ (- 1), respectively. The rats in the metformin group were given metformin 0.15 g 路kg ~ (- 1), and the rats in the normal control group and the model group were given the same volume of purified water. All the rats in each group were given continuously for 4 w. Fasting for 12 hours after the last administration, chloraldehyde hydrate anesthesia, abdominal vein blood samples were taken to detect serum empty abdominal blood glucose, (FBG), insulin, malondialdehyde (MDA), (SOD), catalase (CAT), C) reactive protein (CRP), (CRP),). The levels of tumor necrosis factor-伪 (TNF- 伪) and IL-6 (IL-6) were measured by electromyography. Motor nerve conduction velocity (MNCV) and sensory nerve conduction velocity (SNCV). Were measured by electromyography. Results: compared with the model group, the serum FINS level and IRI of FBG, MNCV and SNCV of sciatica increased significantly (P01), the activity of SOD,CAT increased (P01), and the serum MDA, of rats in high dose group decreased significantly (P01). The contents of CRP,TNF- 伪 and IL-6 decreased significantly (P 0.01). Conclusion: Menghua Powder can improve insulin resistance and peripheral nerve function in diabetic rats, and its mechanism may be related to antioxidation and decrease of inflammatory factor level.
【作者单位】：河北中医学院基础医学院;华北制药新药研究开发公司;
【基金】：河北省科技攻关计划课题(07276101D-7)
【分类号】：R285.5

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