c-junN端蛋白激酶1磷酸化与糖尿病大鼠早期心肌损伤关系研究

发布时间：2018-01-08 19:02

  本文关键词：c-junN端蛋白激酶1磷酸化与糖尿病大鼠早期心肌损伤关系研究　出处：《中国全科医学》2016年27期 　论文类型：期刊论文

  更多相关文章： 糖尿病 心肌疾病 c-jun N端蛋白激酶 瞬时受体电位通道C亚族

【摘要】：目的探讨c-jun N端蛋白激酶1(JNK1)磷酸化与糖尿病(DM)大鼠早期心肌损伤的关系。方法2013年10月—2014年7月,80只SPF级雌性SD大鼠适应性喂养1周后,采用随机数字表法选取60只作为DM模型组,给予高糖高脂饮食饲养4周,一次性左下腹腹腔注射1%链脲佐菌素(STZ)溶液(35 mg/kg);另外20只作为对照组(A组),给予普通饮食饲养4周,一次性左下腹腹腔注射等量枸橼酸缓冲液。最终共48只大鼠造模成功,采用随机数字表法分为B组、C组、D组,各16只。C组大鼠腹腔注射SP600125,D组大鼠皮下埋置含有血管紧张素Ⅱ(AngⅡ)+0.9%氯化钠溶液的植入式胶囊渗透压泵,A组、B组大鼠腹腔注射0.9%氯化钠溶液。饲养6周后,采用随机数字表法从4组大鼠中分别选取10只,检测大鼠心体比(H/B)、左心室质量指数(LVMI),HE染色观察心肌组织形态学改变,免疫组化SABC法检测JNK1、磷酸化JNK1(p-JNK1)、瞬时受体电位通道C亚族(TRPC)6、胶原蛋白Ⅰ(collagenⅠ)表达水平。结果 B组、C组、D组大鼠H/B大于A组,B组、D组大鼠LVMI大于A组(P0.05);C组大鼠H/B、LVMI小于B组、D组(P0.05)。A组大鼠心肌细胞排列整齐,细胞间连接紧密,心肌纹理清晰;B组、C组、D组大鼠心肌细胞均有不同程度的肥大性改变,细胞间隙增宽,心肌细胞排列紊乱,心肌纹理欠清晰。B组、C组、D组大鼠JNK1、p-JNK1、TRPC6、collagenⅠ表达水平高于A组(P0.05);C组大鼠JNK1、p-JNK1、TRPC6、collagenⅠ表达水平低于B组(P0.05);D组大鼠p-JNK1、TRPC6、collagenⅠ表达水平高于B组、C组,JNK1表达水平高于C组(P0.05)。结论高糖环境可诱导JNK1磷酸化,进而影响下游TRPC6信号表达,造成胶原蛋白沉积过多,导致心肌细胞肥大和纤维化,从而引起糖尿病心肌病的发生发展。
[Abstract]:Objective to investigate the relationship between the phosphorylation of c-jun N-terminal protein kinase 1 (JNK1) and early myocardial injury in rats with diabetes mellitus (DM). Methods from October 2013 to July 2014. After one week of adaptive feeding, 60 SPF female SD rats were selected as DM model group and fed with high sugar and high fat diet for 4 weeks. 1% mg / kg of streptozotocin (STZ) solution was injected intraperitoneally into the left lower abdomen. The other 20 rats were fed with general diet for 4 weeks, and the same amount of citric acid buffer was injected into the abdominal cavity of left abdomen. Finally, 48 rats were successfully established. The rats in group B were randomly divided into group C (n = 16) and group C (n = 16). SP600125 was injected intraperitoneally in each group (n = 16). Rats in group D were subcutaneously implanted with angiotensin 鈪，

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