高效液相色谱法测定人血浆中的百草枯

发布时间：2018-01-11 17:25

本文关键词：高效液相色谱法测定人血浆中的百草枯　出处：《现代预防医学》2016年17期 　论文类型：期刊论文

【摘要】：目的建立人血浆中百草枯的高效液相色谱快速测定方法。方法用正交试验优化色谱条件,流动相为甲醇-缓冲液(10:90,v/v),缓冲液为20 mmol/L的十二烷基庚烷磺酸钠(含20 mmol/L磷酸,用三乙胺调节p H为3.5),检测波长:256 nm,流速:0.80 ml/min,柱温为室温。样品依次用乙腈、二氯甲烷处理沉淀血浆中蛋白质并除去脂溶性杂质,取20μl上清液进样测定。结果血浆百草枯在0.1μg/ml到10μg/ml范围内线性关系良好,回归方程为y=6442.6x+44.47(r=0.9992),平均回收率为93.8%-101.1%,日内RSD为3.08%,日间RSD为3.92%。结论该方法简便快速、准确灵敏,适用于人血浆中百草枯快速检测,为百草枯中毒患者的临床诊断提供参考依据。
[Abstract]:Objective to establish a rapid HPLC method for the determination of paraquat in human plasma. Methods the chromatographic conditions of paraquat in human plasma were optimized by orthogonal test. The mobile phase was methanol buffer 10: 90 v / v / v). Sodium 12 alkyl heptane sulfonate (containing 20 mmol/L phosphoric acid) was used as buffer solution for 20 mmol/L. The pH was adjusted to 3.5 渭 m with triethylamine. The detection wavelength was at 1: 256nm. The flow rate was 0.80 ml / min and the column temperature was room temperature. The samples were treated with acetonitrile and dichloromethane to precipitate the protein and remove the lipophilic impurities. Results the linear range of plasma paraquat was 0.1 渭 g / ml to 10 渭 g / ml. The regression equation was YJY 6442.6x 44.47L 0.9992, the average recovery was 93.8-101.1 and the intraday RSD was 3.08%. Conclusion this method is simple, rapid, accurate and sensitive. It is suitable for rapid detection of paraquat in human plasma and provides reference for clinical diagnosis of paraquat poisoning.
【作者单位】：四川大学华西第四医院检验科;四川大学华西公共卫生学院(华西第四医院);四川省绵竹市疾病预防控制中心;
【分类号】：R595.4;R446.1
【正文快照】： 3.四川省绵竹市疾病预防控制中心,四川绵竹,618200百草枯(paraquat),其化学式为C12H14Cl2N2,分子量为257.2,熔点为175-180℃,沸点高于300℃,易溶于水、醇、丙酮等,在酸性及中性溶液中较为稳定,在碱性介质中不稳定,遇紫外线分解[1]。百草枯是世界上用量仅次于草甘膦的除草剂,具

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