酪酸梭菌对食物过敏小鼠肠道屏障功能的影响

发布时间：2018-01-28 05:35

本文关键词： 食物过敏 Trek 肠道屏障功能益生菌　出处：《重庆医学》2017年22期 　论文类型：期刊论文

【摘要】：目的探讨产丁酸的益生菌酪酸梭菌通过调节肠上皮细胞双孔钾通道Trek1的表达对过敏小鼠肠道屏障功能的影响。方法建立小鼠食物过敏模型,ELISA法、流式细胞仪检测相关指标验证造模效果,尤斯室检测小肠组织通透性变化,Western blot及免疫荧光法检测Na6ve组、Saline组、SIT组、SIT/SB组、SB组、SIT/CB组、CB组、SIT/CB/Spadin组小鼠空肠组织中Trek1的表达;Transwell系统中使用T84细胞建立单层上皮细胞层,分别暴露于过敏介质,qRT-PCR和Western blot检测Trek1mRNA和蛋白的表达;分别使用生理盐水、SIT、SIT/SB、SB、SIT/CB、CB、SIT/CB/Spadin不同疗法对过敏小鼠进行治疗,了解小鼠肠道Trek1的表达情况、肠道屏障功能及过敏反应指标的变化情况。结果相对于对照组,食物过敏小鼠小肠Trek1蛋白表达水平显著下降,肠黏膜通透性显著升高,差异有统计学意义(P0.05);T84细胞暴露于过敏介质后,Trek1mRNA和蛋白的表达显著下降(P0.05),但预先加入p38抑制剂可以拮抗这种改变;SIT治疗、酪酸梭菌和丁酸钠单独应用均可以提高食物过敏小鼠肠道Trek1的表达,减轻过敏反应(P0.05),但SIT与酪酸梭菌或丁酸钠的联合应用效果更加显著(与SIT组相比P0.05),并且可以显著降低小肠黏膜通透性,改善肠道屏障功能(P0.05)。结论丁酸钠或者产丁酸的益生菌酪酸梭菌可以通过提高Trek1的表达来恢复过敏小鼠的肠道屏障功能,减轻过敏反应,并且加强SIT治疗的效果。
[Abstract]:Objective to investigate the effects of Clostridium caseinate, a probiotics producing butyric acid, on intestinal barrier function in allergic mice by regulating the expression of double-pore potassium channel Trek1 in intestinal epithelial cells. ELISA and flow cytometry were used to test the effect of model making. The changes of intestinal permeability were detected by Eus chamber. The changes of intestinal permeability were detected by Western blot and immunofluorescence were used to detect the Na6ve group. The expression of Trek1 in jejunum of Saline / sit / sit / SB / SB / CB / CB / CB group. T84 cells were used in the Transwell system to establish a single layer of epithelial cells, which were exposed to allergy mediators respectively. QRT-PCR and Western blot were used to detect the expression of Trek1mRNA and protein. The allergic mice were treated with different treatments of normal saline SITS / SBT / SBT / CBT / CBST / CB/ Spadin. Results compared with the control group, the expression of Trek1 protein in the small intestine of mice with food allergy was significantly lower than that in the control group. The permeability of intestinal mucosa was significantly increased (P 0.05). The mRNA and protein expression of Trek1 in T84 cells decreased significantly after exposure to allergy mediators, but p38 inhibitor could antagonize the changes. SIT treatment, Clostridium caseinate and sodium butyrate alone can increase the expression of Trek1 in the intestine of food allergy mice and alleviate the allergic reaction (P0.05). However, the combination of SIT with Clostridium caseinate or sodium butyrate was more effective (compared with SIT group, P 0.05), and could significantly reduce intestinal mucosal permeability. Conclusion Sodium butyrate or butyric acid-producing probiotics Clostridium caseate can restore the intestinal barrier function of allergic mice by increasing the expression of Trek1 and alleviate the allergic reaction. And enhance the effect of SIT treatment.
【作者单位】：郑州大学第五附属医院消化科;
【基金】：国家自然科学基金资助项目(81370494) 国家973计划前期研究专项(2011CB512006) 河南省科技创新杰出人才基金(134200510022) 郑州市创新型科技人才队伍建设工程基金(131PLJRC656)
【分类号】：R593.1
【正文快照】： 近年来,过敏性疾病的发病率逐渐增高,其中约有8%的儿童和5%的成年人发生过针对某些特定食物抗原的食物过敏。食物过敏是一种Th2细胞因子驱动的免疫紊乱疾病,症状包括腹痛、呕吐、荨麻疹、腹泻等,严重者可发生过敏性休克。肠道屏障功能对于维持机体的免疫稳态有着重要的意义,研

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