高脂饮食喂养建立高脂血症模型的验证及规律探讨

发布时间：2018-01-30 01:34

本文关键词： 高脂血症动物模型高脂饮食　出处：《中国比较医学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的高脂饮食喂养SD大鼠8周建立高脂血症,验证该造模方法的可行性,并探究大鼠在不同时点的血脂变化规律。方法正常雄性8周龄SD大鼠30只,适应性喂养1周后,根据体重随机分为对照组、模型1组、模型2组,每组10只。对照组给予普通饲料,模型组2组喂养高脂高胆固醇饲料,喂养时间为8周,每日计量各组饮食、饮水,4 d更换垫料并称重。分别在第4周、6周、8周末,动物禁食过夜并眼眶静脉丛采血,测定各组血脂四项水平。8周末动物处死,收集各组大鼠肝脏、主动脉进行HE染色。结果与对照组相比,模型1组的日平均饮食量有所增加,但差异无统计学意义;模型2组的日平均饮食量明显减少,差异有统计学意义(P0.01)。日饮水量与对照组相比,模型组2组饮水量明显减少,差异有统计学意义(P0.01)。肝脏/体重指数与对照组相比,模型组2组明显增重,差异有统计学意义(P0.01)。终末体重与对照组相比,模型组2组的体重增加,但只有模型1组有统计学意义(P0.05)。血脂方面,与对照组相比,4周末、6周末模型组2组TC水平明显升高,差异有统计学意义(P0.01或P0.05),8周末模型1组LDL-c水平明显升高,HDL-c水平降低,差异有统计学意义(P0.05)。肝脏HE染色示对照组肝细胞排列正常,染色均匀,而模型组2组肝细胞大面积脂肪变,少数肝血窦内充血及炎症细胞浸润。主动脉HE染色示3组主动脉结构正常。结论高脂饲料喂养SD大鼠建立高脂血成立,所造模型为高胆固醇血症并伴有严重的脂肪肝。同时,高脂高胆固醇饲料喂养SD大鼠形成高脂血症实验过程中,血脂波动呈现一个升高-内适应-升高的状态。在造模过程中,如何克服动物厌食并胆固醇代谢内调节这个问题,是高脂饲料喂养成模的关键所在。
[Abstract]:The purpose of high fat diet fed SD rats for 8 weeks to establish hyperlipidemia, verify the feasibility of the modeling method, and explore the changes of blood lipid in rats at different time points. The method of the normal 8 week old male SD 30 rats, after 1 weeks of feeding, were randomly divided into control group, model 1 group 2, model group, 10 rats in each group. The control group was given normal diet, model group 2 group were fed with high cholesterol and high fat diet feeding for 8 weeks, daily measurement each diet, drinking water, 4 d replace the packing and weighing respectively. In fourth weeks, 6 weeks, 8 weeks, overnight fasting and animal eyes blood lipids were measured by venous plexus, four level.8 weekend animal death, liver was collected in rat aortic HE staining. Results compared with the control group, model group 1 average daily diet increased, but the difference was not statistically significant; the model 2 groups of average daily diet decreased significantly, with statistical difference Significance (P0.01). Compared with the control group, drinking water, drinking water model group 2 groups were significantly decreased, the difference was statistically significant (P0.01). The liver / body weight index compared with the control group, model group, the 2 group was increasing, the difference was statistically significant (P0.01). The final body weight compared with the control group. Model 2 groups of weight gain, but only the model 1 group was statistically significant (P0.05). Blood lipid, compared with the control group at the end of the 4, 6 weekend model group TC levels in the 2 groups increased significantly, the difference was statistically significant (P0.01 or P0.05), at the end of the 8 week model group 1 LDL-c level significantly increased, HDL-c the level is reduced, the difference was statistically significant (P0.05). The liver HE staining showed that the liver cells arranged in normal control group and model group, dyeing uniformity, large area of 2 groups of liver cell fatty degeneration, inflammatory cell infiltration and congestion of liver blood sinus. Few aortic HE staining showed that 3 groups of active pulse structure was normal. Conclusion high fat diet SD rats with hyperlipidemia was established, the model for hypercholesterolemia and severe fatty liver. At the same time, high fat and high cholesterol diet in SD rats with hyperlipidemia in the course of the experiment, blood lipid fluctuations showed a rise in adaptation to elevated status. In the modeling process, how to overcome the regulation of cholesterol metabolism in animal and anorexia, is high fat feed into key modules.

【作者单位】：广州中医药大学基础医学院;
【基金】：国家重点基础研究发展计划(2014CB542901)
【分类号】：R589.2;R-332
【正文快照】： 高脂血症是临床常见疾病,也是导致脑卒中、冠心病、心脏猝死独立而重要的危险因素[1]。制作高脂血症的动物模型对于研究代谢性疾病及心脑血管疾病意义重大。高脂血症动物模型制作有多种方法,常见的有高脂高胆固醇饲料喂养[2]、脂肪乳灌胃[3]、氢化可的松肌肉注射[4]、Triton W

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