B细胞活化因子的双向性:促进调节性T细胞分泌白细胞介素-35

发布时间：2018-01-31 17:21

本文关键词： 系统性红斑狼疮 B细胞活化因子调节性T细胞白细胞介素-　出处：《首都医科大学学报》2017年05期 　论文类型：期刊论文

【摘要】：目的研究B细胞活化因子(B cell activation factor of the tumor necrosis factor family,BAFF)对调节性T细胞(Tregs)分泌抑制性细胞因子白细胞介素-35(interleukin-35,IL-35)的影响,验证系统性红斑狼疮(systemic lupus erythematosus,SLE)中BAFF对免疫反应的双向调节机制。方法磁珠分选狼疮模型鼠Tregs,经BAFF刺激后采用qRT-PCR及流式细胞术检测其分泌IL-35浓度。比较正常对照鼠、狼疮模型鼠及经尾静脉注射抗BAFF蛋白后狼疮模型鼠脾脏Tregs频率及分泌IL-35情况。结果BAFF能够有效促进Tregs分泌IL-35。相对于正常对照鼠,狼疮模型鼠(血清中BAFF升高)脾脏Tregs分泌较高浓度的IL-35,而中和其循环中BAFF后,Tregs分泌IL-35减少。结论在SLE发病机制中,BAFF不仅是重要的致病因素,还能够通过促进Tregs细胞分泌IL-35发挥负向免疫调节效应。
[Abstract]:Objective to study B cell activating factor (B cell activating factor). B cell activation factor of the tumor necrosis factor family. The effect of BAFF on the secretion of interleukin-35 interleukin-35 (IL-35), an inhibitory cytokine (IL-35) in regulatory T cells (Tregs). To verify systemic lupus erythematosus systemic lupus erythematosus. Methods the Tregs of lupus model mice was separated by magnetic beads. The concentration of IL-35 secreted by BAFF was detected by qRT-PCR and flow cytometry. The frequency of Tregs and the secretion of IL-35 in the spleen of lupus model rats and mice injected with BAFF protein via caudal vein were analyzed. Results BAFF could effectively promote the secretion of IL-35 by Tregs. Compared with normal control mice. High concentration of IL-35 was secreted by Tregs in the spleen of lupus model rats (elevated BAFF in serum) and was neutralized after circulating BAFF. Conclusion Tregs is not only an important pathogenic factor in the pathogenesis of SLE. It can also play a negative immunomodulatory effect by promoting the secretion of IL-35 by Tregs cells.
【作者单位】：华中科技大学同济医学院附属协和医院皮肤科;
【基金】：国家自然科学基金(81573047,81602760)~~
【分类号】：R593.241
【正文快照】： 系统性红斑狼疮(system lupus erythematosus,SLE)是一种复杂的、累及全身多脏器的系统性自身免疫性疾病[1-2]。B细胞活化因子(B cell activation fac-tor of the tumor necrosis factor family,BAFF)是对自身反应性B细胞增生分化至关重要的关键细胞因子。在SLE病人的血浆中BA

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