血清VEGF及ICAM-1水平与糖尿病视网膜病变患者微血管损伤的关系

发布时间：2018-01-31 20:30

  本文关键词： 糖尿病视网膜病变 微血管损伤 血管内皮生长因子 细胞间黏附分子-　出处：《中国老年学杂志》2017年08期 　论文类型：期刊论文

【摘要】：目的探讨血清中血管内皮生长因子(VEGF)及细胞间黏附分子(ICAM)-1水平与糖尿病视网膜病变(DR)患者微血管损伤之间的关系。方法未应用抗凝类药物治疗的2型糖尿病(T2DM)患者120例,根据临床及眼底检查确诊将其分为非DR(NDR)组(n=56)、非增生型DR(NPDR)组(n=30)、增生型DR(PDR)组(n=34)。比较各组微血管病变发生率;采用流式细胞仪检测内皮细胞(ECs)、内皮祖细胞(EPCs)计数百分比;采用酶联免疫吸附法(ELISA)检测各组血清中VEGF及ICAM-1水平。结果 PDR组糖尿病肾病(DN)及神经病变的发生率明显高于NDR组和NPDR组(均P0.05);与未发生微血管病变患者相比,发生微血管病变患者的EPCs水平明显下降,而ECs、VEGF和ICAM-1水平均明显升高(均P0.05);不管是否发生微血管病变,PDR组的ECs、VEGF和ICAM-1水平均高于NDR组和NPDR组,NPDR组均高于NDR组,PDR组EPCs水平低于NPDR组,NPDR组低于NDR组(均P0.05);PDR组VEGF、ICAM-1水平与ECs水平呈正相关(r=0.995,0.852,P0.05),与EPCs水平呈负相关性(r=-0.895,-0.920,P0.05)。结论 VEGF和ICAM-1在DR患者微血管损伤中高表达,与ECs和EPCs密切相关,提示VEGF和ICAM-1在DR患者微血管损伤形成中与血管内皮功能密切相关。
[Abstract]:Objective to investigate the levels of vascular endothelial growth factor (VEGF), intercellular adhesion molecule (ICAM-1) and diabetic retinopathy (DRR) in serum. Methods 120 patients with type 2 diabetes mellitus (T2DM) without anticoagulant therapy were studied. According to the diagnosis of clinical and fundus examination, the patients were divided into two groups: non-DRN NDR group (n = 56) and non-proliferative DRN group (n = 30). The incidence of microvascular lesions in the proliferative DRV PDR group was compared. Flow cytometry was used to detect the percentage of ECsN and EPCs in endothelial progenitor cells. The levels of VEGF and ICAM-1 in serum of each group were detected by enzyme linked immunosorbent assay (Elisa). Results the serum levels of VEGF and ICAM-1 in PDR group were determined by diabetic nephropathy. The incidence of neuropathy in NDR group and NPDR group was significantly higher than that in NDR group and NPDR group (P 0.05). Compared with those without microvascular disease, the level of EPCs in patients with microvascular disease was significantly decreased, while the levels of ICAM-1 and ICAM-1 were significantly increased in patients with microvascular disease (all P 0.05). The levels of ICAM-1 and ICAM-1 in PDR group were higher than those in NDR group and NPDR group. The level of EPCs in PDR group was lower than that in NPDR group and that in NDR group was lower than that in NDR group (all P 0.05). In PDR group, the level of VEGF ICAM-1 was positively correlated with the level of ECs. There was a negative correlation between VEGF and EPCs level. Conclusion VEGF and ICAM-1 are highly expressed in Dr patients with microvascular injury. It is suggested that VEGF and ICAM-1 are closely related to vascular endothelial function in the formation of microvascular injury in Dr patients.
【作者单位】： 中山大学中山眼科中心海南眼科医院眼底病专科;中山大学中山眼科中心海南眼科医院眼底外科;中山大学中山眼科中心海南眼科医院准分子激光中心;
【分类号】：R587.2;R774.1
【正文快照】： 糖尿病常见的血管并发症糖尿病视网膜病变(DR)的发病机制和病因不明确,其典型特点包括新生血管过度增殖、血管内皮细胞功能异常以及微血管瘤等病征。血管内皮生长因子(VEGF)属于促血管生长因子的一种,其生理功能主要是推进血管内皮细胞的增殖与迁移,以达到维持血管通透性和完，

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