慢性高原病大鼠肝组织中扭转原肠胚形成同系物1、铁调素表达观察

发布时间：2018-02-15 07:57

  本文关键词： 慢性高原病 扭转原肠胚形成同系物 铁调素　出处：《山东医药》2017年15期 　论文类型：期刊论文

【摘要】：目的观察慢性高原病(CMS)大鼠肝组织中扭转原肠胚形成同系物1(TWSG1)、铁调素的表达变化,并探讨其意义。方法 50只雄性健康大鼠由西安运至西宁,随机分成6组,即低氧1组、低氧2组、CMS组、常氧1组、常氧2组、常氧3组。常氧1、2、3组分别在西宁(海拔2 260 m)常规饲养7、15、30 d。低氧1、2组和CMS组放入低压氧仓模拟海拔5 000 m环境,分别饲养7、15、30 d。CMS组成功制作CMS模型。采用qRT-PCR法检测肝组织中的铁调素、TWSG1 mRNA;采用免疫组化法检测肝组织中的TWSG1蛋白。结果低氧2组、CMS组肝组织中铁调素mRNA相对表达量分别低于常氧2、3组,CMS组低于低氧1组(P均0.05)。低氧1组、低氧2组、CMS组肝组织中TWSG1 mRNA相对表达量分别高于常氧1、2、3组,低氧1组、低氧2组、CMS组TWSG1 mRNA相对表达量依次增高(P均0.05)。低氧1组、低氧2组、CMS组TWSG1蛋白表达阳性分别为8、9、9例,常氧1、2、3组分别为8、8、8例。低氧2组、CMS组肝组织中TWSG1蛋白相对表达量分别高于常氧2、3组,低氧1组、低氧2组、CMS组TWSG1蛋白相对表达量依次增高(P均0.05)。结论 CMS大鼠肝组织中铁调素表达下调,TWSG1表达上调;随低氧暴露时间延长,机体铁调素和TWSG1表达变化越明显,预示CMS状态下铁代谢逐渐发生改变。
[Abstract]:Objective to observe the changes of the expression of TWSG1 and iron in the liver tissue of rats with chronic mountain sickness (CMSM). Methods 50 healthy male rats were randomly divided into 6 groups, namely hypoxia group 1, from Xi'an to Xining. Hypoxia group 2: CMS group, normoxic group 1, normoxic group 2, normoxic group 3. Normoxic 1o 2 group was fed in Xining (2 260 m above sea level) for 7 15 ~ 30 days respectively. Hypoxia 1 + 2 group and CMS group were placed in a low pressure oxygen chamber to simulate 5 000 m above sea level. The CMS model was successfully made by feeding 7 ~ 1530 d.CMS group. The iron modulin TWSG1 mRNAs in liver tissue were detected by qRT-PCR method, and the TWSG1 protein in liver tissue was detected by immunohistochemical method. Results the relative expression of iron modulin mRNA was detected in liver tissue of hypoxia 2 groups. The difference between CMS group and hypoxia group (P < 0.05) was lower than that in normal oxygen group (P < 0.05), while that in hypoxia group 1 was lower than that in control group (P < 0.05). The relative expression of TWSG1 mRNA in liver tissue of hypoxia 2 group was higher than that of normoxic 1 1 group, hypoxia 1 group and hypoxia 2 group (P < 0 05). The positive expression of TWSG1 protein in hypoxia group and hypoxia 2 group were 89 cases, respectively. The relative expression of TWSG1 protein in liver tissue of hypoxia group 2 was higher than that of normoxic group 2 and hypoxia group 1, respectively. Conclusion the expression of iron modulin in liver tissue of CMS rats is down-regulated and the expression of TWSG1 is up-regulated, and the changes of iron modulin and TWSG1 expression are more obvious with the prolongation of hypoxia exposure time. It is indicated that iron metabolism changes gradually in CMS state.
【作者单位】： 淄博矿业集团集团中心医院;青海大学附属医院;
【基金】：青海省科技计划资助项目(2014-ZJ-720)
【分类号】：R594.3
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本文编号：1512800