IL-22在类风湿关节炎中的表达及临床意义

发布时间：2018-02-21 08:22

本文关键词： 关节炎类风湿白细胞介素疾病活动性骨侵蚀　出处：《中国免疫学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的:探讨RA患者IL-22的表达水平并分析其临床意义。方法:流式细胞术检测RA患者(50例)、OA患者(15例)、健康对照组(15例)外周血及RA关节滑液Th22细胞所占比例,ELISA法检测血清及RA关节滑液中IL-22水平。检测RA患者疾病活动性、临床指标、骨破坏情况,分析其与IL-22水平相关性。两组间比较用t检验,相关性分析采用Pearson直线相关分析法,多组样本比较用Kruskal-Wallis H检验。结果:RA患者外周血中Th22细胞比例高于OA组(t=2.290,P=0.021)及健康对照组(t=2.524,P=0.015);RA患者血清IL-22水平高于OA患者(t=2.560,P=0.014)及健康对照组(t=2.768,P=0.009)。RF阳性RA血清IL-22水平高于RF阴性RA(t=2.322,P=0.035)。抗CCP抗体阳性RA血清IL-22水平高于抗CCP抗体阴性RA(t=2.504,P=0.015)。RA血清IL-22水平与ESR、RF、DAS28评分呈正相关关系(r分别为0.312、0.314、0.332,P0.05)。RA患者血清IL-22水平随骨侵蚀的加重呈递增趋势,X线不同分期之间差异有统计学意义(H=9.14,χ_(0.05(3))~2=7.81,Hχ_(0.05(3))~2,P0.05)。有关节积液RA患者血清IL-22水平高于无关节积液RA患者(t=2.587,P=0.012),关节滑液IL-22水平高于血清(t=2.668,P=0.011),与关节滑液Th22细胞比例无相关性(r=0.287,P=0.065)。结论:RA患者血清及关节滑液IL-22水平增高,与疾病活动性及骨破坏相关,可成为病情评估与骨破坏的指标。IL-22可能为RA治疗的新靶点。
[Abstract]:Objective: To investigate the expression level of RA in patients with IL-22 and evaluate its clinical significance. Methods: the patients with RA were detected by flow cytometry (50 cases), OA patients (15 cases), control group (15 cases) in peripheral blood and synovial fluid RA the percentage of Th22 cells, ELISA assay of serum and synovial RA the level of IL-22 in liquid. Clinical indexes of RA disease activity, and bone destruction, analyze its correlation with the level of IL-22. The two groups using t test, correlation analysis using Pearson correlation analysis, multiple samples were compared with Kruskal-Wallis H test. Results: RA patients with Th22 cell ratio in peripheral blood higher than that of group OA (t=2.290, P=0.021) and control group (t=2.524, P=0.015); the serum IL-22 level in patients with RA than patients with OA (t=2.560, P=0.014) and control group (t=2.768, P=0.009) level of serum IL-22 RA.RF positive than RF negative RA (t=2.322, P=0.035). Anti CCP antibody positive serum RA IL The level of -22 is higher than that of negative anti CCP antibody RA (t=2.504, P=0.015).RA serum IL-22 level and ESR, RF, DAS28 scores were positively correlated (r = 0.312,0.314,0.332, P0.05) of serum IL-22 levels in patients with.RA with bone erosion aggravate X-ray showed an increasing trend, different stages have statistically significant difference (H=9.14, _ (0.05 x (3) ~2=7.81, H (_) was 0.05 (3)) ~2, P0.05). The serum IL-22 level in patients with joint effusion was higher than that of RA without joint effusion in patients with RA (t=2.587, P=0.012), higher serum IL-22 levels in synovial fluid (t=2.668, P=0.011), and no correlation between synovial cell ratio (r=0.287, P=0.065 Th22). Conclusion: the expression of RA in serum and synovial fluid levels of IL-22 correlated with disease activity and bone destruction, may become a new target index of.IL-22 severity and bone destruction for RA treatment.

【作者单位】：重庆市中医院风湿病科;
【基金】：重庆市卫计委医学科研项目(2015MSXM080)
【分类号】：R593.22

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