谷氨酰胺对热打击下单层肠上皮Caco-2细胞屏障功能的影响

发布时间：2018-03-01 14:15

  本文关键词： 热打击 谷氨酰胺 肠黏膜　出处：《解放军医学杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的探讨谷氨酰胺(Gln)对热打击后肠黏膜单层上皮细胞屏障通透性改变的影响及可能机制。方法应用Caco-2细胞株建立肠上皮机械屏障模型,加入Gln培养24h,43℃持续1h热打击。以CCK-8法检测不同浓度Gln(0.4、0.7、1.4、2.1、2.8mmol/L)对细胞增殖的影响,为后续实验选择最适合浓度;Transwell法测定单层跨膜电阻抗(TEER)值和对辣根过氧化物酶(HRP)的通透性;Western blotting检测紧密连接蛋白occludin和ZO-1的表达;采用考马斯亮蓝对细胞骨架进行染色观察。结果 0.7mmol/L的Gln促进细胞增殖的效果最强,与其他浓度相比,差异均有统计学意义(P0.05)。相较于单纯43℃热打击组,0.7mmol/L的Gln可抑制单层上皮细胞TEER的下降和HRP通过率的升高(P0.01),增加occludin和ZO-1的表达,有益于维持细胞骨架的正常结构。结论 0.7mmol/L的Gln可减轻热打击对单层肠上皮细胞结构的破坏,保护屏障功能。
[Abstract]:Objective to investigate the effect of glutamine (Glutamine) on the permeability of intestinal mucosal monolayer epithelial cell barrier after thermal shock and its possible mechanism. Methods Caco-2 cell line was used to establish the model of intestinal epithelial mechanical barrier. The effects of different concentrations of Glnn on cell proliferation were detected by CCK-8 assay. The effects of different concentrations of Glnn on cell proliferation were measured by CCK-8 assay at 43 鈩，

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