5-氨基水杨酸对百草枯中毒大鼠肾脏的保护作用及机制

发布时间：2018-03-04 19:52

本文选题：5-氨基水杨酸　切入点：百草枯　出处：《遵义医学院》2017年硕士论文　论文类型：学位论文

【摘要】：目的:探讨5-氨基水杨酸对百草枯中毒大鼠肾脏的保护作用及机制。方法:将144只雄性SD大鼠随机分为4组:空白对照组(N组)、5-ASA对照组(A组)、PQ中毒组(P组)和5-ASA治疗组(P+A组),P组与P+A组采用2%PQ溶液腹腔注射建立急性肾损伤模型,其余两组给予生理盐水腹腔注射;A组与P+A组腹腔注射2h后给予5-ASA(75mg/kg)溶液一次性灌胃,最长用药时间为7天,P组与N组则灌入等量生理盐水;分别于6h、12h、24h、72h、168h麻醉后心脏采血,检测肾损伤标志物Scr、BUN,氧化损伤标志物SOD、CAT、GSH、MDA含量,留取肾组织行病理切片及Nrf2、HO-1蛋白含量的监测。结果:1、P组与P+A组大鼠染毒后出现行为学改变及病理损伤,但P+A组较P组明显减轻,其余两组未出现中毒表现。2、P组BUN值与N组比较从12h开始明显增高,在12h,24h,72h,168h升高明显(P0.01),Cr值与N组比较从24h开始明显升高,在24h,72h,168h升高明显(P0.05),经5-ASA治疗后,P+A组BUN值在12h,24h,72h,168h均较P组明显下降(P0.01),Cr值在24h,72h较P组明显下降(P0.01)。3、氧化反应标志物:A组与N组在各个时间点血清SOD活力、MDA、CAT、GSH含量均维持在相对稳定水平,P组SOD活力及GSH、CAT含量在染毒后12h,24h,72h,168h与N组比较均明显下降(P0.05),P+A组与P组比较在第24h,72h,168h明显上升(P0.05);P组染毒后6h即出现MDA含量升高,在12h,24h,72h,168h明显升高(P0.05);P+A组与P组相比较在第24h,72h,168h下降明显(P0.05)。4、Nrf2及HO-1含量:与N组比较,P组Nrf2及HO-1蛋白含量在染毒后24h及72h均升高,24h升高明显(P0.05),P+A组Nrf2及HO-1蛋白含量与P组比较在24h,72h均明显升高(P0.01);A组与N组比较肾组织中Nrf2及HO-1蛋白含量明显上升,差异具有统计学意义(P0.01)。结论:5-ASA可减轻PQ导致的急性肾损伤,其机制可能与激活Nrf2-ARE信号通路有关。
[Abstract]:Objective: to investigate the protective effect and mechanism of 5-aminosalicylic acid on the kidney of paraquat poisoned rats. Methods: 144 male SD rats were randomly divided into four groups: control group (control group), control group (control group), group A (group A) and group P (group P). The acute renal injury model was established by intraperitoneal injection of 2PQ solution in the treatment group. The other two groups were intraperitoneally injected with normal saline, group A and group A were given intraperitoneal injection of 5-ASAA 75mg / kg for 2 hours. The longest time of administration was 7 days after injection of normal saline in group P and group N, and the heart blood was collected after anesthesia at 6h, 12h, 24h, 72h, 168h, respectively. The renal injury marker Scr-BUNand the content of GSHMDA in oxidative damage marker SOD- CATN, and the pathological sections of renal tissue and the protein content of Nrf2HHO-1 were detected. Results the behavioral changes and pathological damage were observed in rats of group 1: 1 and group P. However, the value of BUN in the other two groups was significantly lower than that in the control group. The BUN value in the other two groups was significantly higher than that in the N group from 12h to 12h, and the value of P0.01C increased significantly at 12h, 24h, 72h and 168h, compared with that of the N group. After 5-ASA treatment, the BUN value in group A was significantly lower than that in group P at 12 h, 24 h and 72 h after 5-ASA treatment. Compared with group P at 24 h and 72 h, Cr decreased significantly (P 0.01 路3) compared with group P at 72 h. The activity of serum SOD activity in group A and group N were maintained in phase at all time points. The activity of SOD and the content of GSH-cat in P group were significantly lower than those in group N at 12 h, 24 h or 72 h, 168h after exposure. Compared with group P, the content of MDA in group A was significantly higher than that in group P at 24 h, 72h, 168h, and 6h, respectively. The protein content of Nrf2 and HO-1 in P group was significantly higher than that in group P group at 24 h and 72 h, respectively. The protein contents of Nrf2 and HO-1 in P group were significantly higher than those in group P and P group, and the protein contents of Nrf2 and HO-1 in group P and group P were significantly higher than those in group P at 24 h and 72 h after exposure, and the protein contents of Nrf2 and HO-1 in group P and P were significantly higher than those in group P at 24 h and 72 h, respectively. The protein contents of Nrf2 and HO-1 in renal tissue of group A and group N were significantly higher than those of group N at 24 h or 72 h. Conclusion 5-ASA can attenuate acute renal injury induced by PQ, and its mechanism may be related to activation of Nrf2-ARE signaling pathway.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R595.4

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