牙周干预对Ⅱ型糖尿病牙周炎大鼠颈动脉血管及血清IL-6水平的影响

发布时间：2018-03-11 01:15

本文选题：牙周炎　切入点：Ⅱ型糖尿病　出处：《山西医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的通过观察大鼠的颈动脉血管病理变化,比较血清炎症因子白细胞介素-6(Interleukin-6,IL-6)水平变化,研究牙周干预对Ⅱ型糖尿病(T2DM)牙周炎(chronic periodontitis,CP)大鼠的影响?。方法44只清洁级大鼠,随机分为A组(对照组,7只)、B组(T2DM组,8只)、C组(CP组,14只)、D组(T2DM+CP组,15只)。据是否进行牙周干预,再将C、D组随机分为:C1/D1不干预组、C2/D2牙周干预治疗组,7只/组。通过内眦静脉的采血方法,在干预前1周(T1)、第一次干预后1周(T2)、第二次干预后1周(T3)、3周(T4)、5周(T5)五个时间点采血,干预前后的血清炎症因子IL-6的含量用酶联免疫吸附法(ELISA)来检测,颈动脉血管在制成石蜡切片后,HE染色,光镜下观察变化情况。结果1.颈动脉病理结果显示:A组血管形态正常;B组(T2DM组)多数颈动脉管壁显著增厚,内膜表面富含炎症细胞,较多的空泡性变,纤维排列杂乱;C/D组颈动脉血管壁形态变化程度不一,其中C1/D1不治疗组变化较大,管壁增厚明显,泡沫、炎症细胞广泛的黏附存在于管壁,弹性纤维紊乱,且D1组变化程度更大;而C2/D2牙周干预组的颈动脉管壁厚度不同,局部少量的泡沫细胞,炎症细胞数量也较少,纤维排列有改善,而C2组颈动脉血管状态改善更明显;2.血清学结果:B组IL-6含量在观测期间是呈持续增高的走势(P0.001);C1、D1组的IL-6在观测期间也是持续上升的走势,且IL-6含量在最后时间点时分别高至(C1/D1:337.985±35.866/487.724±18.831,pg·L-1,P0.01);且D1组IL-6含量在干预后时间点明显高于C1组(P0.01);与干预前各组基线相比,干预组C2、D2(除C2组第三个时间点)IL-6的含量在干预后,随时间的变化是呈先升后降的趋势,在干预后1周升高,此时含量分别为(C2:290.212±20.218/D2:656.802±54.232,pg·L-1),并在第三个时间点开始下降(P0.05);且C2/D2牙周干预组IL-6含量干预后时间点,均低于不治疗组C1/D1组相比(P0.01,除第三个时间点外);结论1.IL-6不仅参与了慢性牙周炎及T2DM的发生发展,且可能与血管相关病变的发生发展有关。2.若不及时控制慢性牙周炎症,则可能会加重CP及T2DM的病变程度,促进CP及T2DM血管病变的形成及进展;3.牙周干预短期内可能会增强机体炎症状态,增加颈动脉血管发生病变的风险,而长远效果来看则有可能会降低机体炎症,而改善颈动脉血管的病变程度;
[Abstract]:Objective to study the effect of periodontal intervention on chronic periodontitis (CPP) rats by observing the pathological changes of carotid artery in rats and comparing the changes of serum inflammatory factor interleukin-6 (IL-6). Methods Forty-four clean rats were randomly divided into two groups: group A (control group, n = 7), T2DM group (n = 8), group C (n = 14), group C (n = 14), group D (n = 14), and group D (n = 15) with T2DM. Group D was randomly divided into two groups: the control group (n = 7) and the control group (n = 7). Blood samples were collected from the inner canthus vein at 1 week before intervention, 1 week after the first intervention, 1 week after the first intervention, 1 week after the second intervention, 1 week after the second intervention, 3 weeks after the second intervention, 5 weeks after the intervention, and 5 weeks after the intervention, the blood samples were collected at five time points: 1 week before the intervention, 1 week after the first intervention, 1 week after the first intervention, 1 week after the second intervention, 1 week after the intervention, 5 weeks after the intervention, the blood samples were collected. The content of serum inflammatory factor IL-6 was detected by Elisa before and after intervention. The carotid artery was stained with HE after paraffin section. Results 1. The pathological results of carotid artery showed that the wall of most carotid arteries was thickened significantly, and the surface of intima was rich in inflammatory cells and vacuolar degeneration. 2. In C / D group, the changes of carotid artery wall morphology were different. In C 1 / D group, the changes of carotid artery wall were larger, the wall thickened obviously, foam and inflammatory cells were widely adhered to the wall, the elastic fiber was disordered, and the change degree of D1 group was greater than that of the control group. In the C _ 2 / D _ 2 periodontal intervention group, the thickness of carotid artery wall was different, a small number of foam cells were localized, the number of inflammatory cells was also relatively small, and the arrangement of fibers was improved. The results of serology showed that the content of IL-6 in group C _ 2 was increasing continuously during the observation period. The IL-6 of group C _ (1) C _ (1) in C _ (1) C _ (1) C _ (1) C _ (1) C _ (1) C _ (1) C _ (1) C _ (1) was also increasing during the observation period. The content of IL-6 in group D1 was significantly higher than that in group C1 at the last time point, and the content of IL-6 in group D1 was significantly higher than that in group C1 at the time point after intervention, and compared with the baseline before and after intervention, the content of IL-6 in intervention group was higher than that in group C _ 2 (except the third time point of C2 group), the content of IL-6 in group C _ 2 was significantly higher than that in group C _ 1 at the time point after intervention, and the level of IL-6 in group C _ 2 was significantly higher than that in group C _ 1 at the end of the intervention, and the level of IL-6 in group C _ 2 was significantly higher than that in group C _ 1. The content of IL-6 in the C 2 / D2 periodontal intervention group increased at 1 week after intervention, and the content was 290.212 卤20.218D 2: 656.802 卤54.232PG 路L -1, and decreased at the third time point, and the IL-6 content in the C 2 / D2 periodontal intervention group decreased at the time point after the intervention. Conclusion 1. IL-6 is not only involved in the occurrence and development of chronic periodontitis and T2DM, but also may be related to the occurrence and development of vascular related diseases. It may aggravate the pathological degree of CP and T2DM, promote the formation and progression of CP and T2DM vascular lesions 3.periodontal intervention may enhance the inflammatory state of the body and increase the risk of carotid artery angiopathy in a short period of time. In the long run, it is possible to reduce inflammation and improve the degree of carotid artery disease.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R781.4;R587.1

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