白藜芦醇对哮喘小鼠气道重塑的抑制作用

发布时间：2018-03-17 14:52

本文选题：哮喘　切入点：气道重构　出处：《中国呼吸与危重监护杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的观察白藜芦醇(RV)对慢性哮喘小鼠模型气道重塑的影响。方法 24只雌性BALB/c小鼠随机分成3组,即对照组、哮喘组、RV组,每组8只,3组小鼠均给予鸡卵清蛋白(OVA)致敏,对照组予以磷酸盐缓冲溶液(PBS)激发,哮喘组和RV组予以OVA激发,其中RV组于每次激发前30 min给予腹腔注射溶于二甲基亚砜(DMSO)的RV,对照组和哮喘组给予腹腔注射等量的DMSO。最后一次激发24 h后,取小鼠肺组织固定、切片行过碘酸希夫(PAS)染色、Masson染色及α-平滑肌肌动蛋白(α-SMA)免疫组织化学检测,分别检测杯状细胞增生、胶原沉积和平滑肌细胞增生肥大程度,结果用杯状细胞(PAS阳性)、胶原(Masson阳性)和平滑肌细胞(α-SMA阳性)阳染面积/支气管基底膜周长(μm2/μm)表示。结果对照组未见PAS+区域,哮喘组和RV组杯状细胞增生显著高于对照组(5.44±1.13、4.18±0.85比0.00±0.00,P均0.01),且RV组显著低于哮喘组(P0.05)。Masson染色提示哮喘组和RV组上皮下胶原沉积显著高于对照组(9.80±2.78、5.71±0.68比1.67±0.65,均P0.01),且RV组显著低于哮喘组(P0.01)。α-SMA免疫组化显示哮喘组和RV组上皮下胶原沉积显著高于对照组(10.39±1.65、7.57±1.98比2.41±1.06,均P0.01),且RV组显著低于哮喘组(P0.05)。结论 RV干预后,小鼠慢性哮喘模型气道重塑指标均有减轻,提示RV可能对慢性哮喘小鼠气道重塑具有抑制作用。
[Abstract]:Objective to observe the effect of resveratrol on airway remodeling in chronic asthmatic mice. Methods 24 female BALB/c mice were randomly divided into three groups: control group and asthma group. The control group was stimulated by phosphate buffer solution (PBSs), and the asthma group and RV group were stimulated by OVA. RV dissolved in dimethyl sulfoxide (DMSO) was injected intraperitoneally in RV group 30 min before each challenge, and DMSO was injected intraperitoneally in control group and asthma group. 24 hours after the last challenge, lung tissue was fixed in mice. The sections were examined by Masson staining with Schiff's acid and immunohistochemistry with 伪 -SMA.The goblet cell proliferation, collagen deposition and hypertrophy of smooth muscle cells were detected. Results the positive area of pas, collagen and smooth muscle cells (伪 -SMA positive) and the area of bronchobasilar membrane circumference (渭 m2 / 渭 m) were expressed by goblet cell pas positive, collagen Masson positive and 伪 -SMA positive. Results there was no PAS region in the control group. The goblet cell proliferation in asthma group and RV group was significantly higher than that in control group (5.44 卤1.13 卤4.18 卤0.85 vs 0.00 卤0.00P 0.01, P < 0.01), and the staining of epiglotous collagen in RV group was significantly lower than that in asthma group and RV group, which indicated that the deposition of epiglotous collagen in asthma group and RV group was significantly higher than that in control group (9.80 卤2.785.71 卤0.68 vs 1.67 卤0.65, P 0.01), and that in RV group was significantly lower than that in RV group. 伪 -SMA immunohistochemical staining showed that the accumulation of epigastric collagen in asthma group and RV group was significantly higher than that in control group (10.39 卤1.65U 7.57 卤1.98 vs 2.41 卤1.06), and that in RV group was significantly lower than that in asthma group (P 0.05). The indexes of airway remodeling in mice model of chronic asthma were alleviated, suggesting that RV may inhibit airway remodeling in mice with chronic asthma.
【作者单位】：武汉大学中南医院呼吸内科;
【基金】：国家自然科学基金面上项目(81072684) 湖北省自然科学基金面上项目(2014CFB202)
【分类号】：R562.25

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