二苯乙烯苷对糖尿病大鼠心肌损伤的保护作用

发布时间：2018-03-18 02:21

  本文选题：糖尿病心肌病　切入点：二苯乙烯苷　出处：《中国药理学通报》2016年03期 　论文类型：期刊论文

【摘要】：目的探讨二苯乙烯苷(TSG)对糖尿病大鼠心肌损伤的作用及对沉默信息调节因子2的哺乳动物同源体1(SIRT1)和磷酸腺苷活化的蛋白激酶(AMPK)蛋白影响。方法建立2型糖尿病大鼠模型,分组给药,16周时处死大鼠,生化法测定血糖、血脂、肝功能及肌酸激酶(CK)、乳酸脱氢酶(LDH)和心肌组织游离脂肪酸(NEFA);酶联免疫吸附法测定心肌肌钙蛋白Ⅰ(cTnⅠ)、心肌组织脂代谢相关酶脂肪酸跨膜转运载体蛋白(FATPs)和脂肪酸β氧化酶(FA-β-oxidase)的含量;放射免疫法测定血浆炎症因子肿瘤坏死因子(TNF-α)、白细胞介素6(IL-6)、白细胞介素1β(IL-1β)的含量,Western blot检测心肌组织中TNF-α、IL-6、IL-1β、SIRT1和AMPK蛋白的表达;并测定左心室胶原含量。结果TSG干预后能减低血脂水平,对血糖和胰岛素水平无明显影响。TSG能减低糖尿病大鼠心肌组织中胶原含量,减少心脏游离脂肪酸含量,增加心肌组织脂代谢相关酶(FATPs和FA-β-oxidase)含量,抑制外周血和心肌组织中炎症因子(TNF-α、IL-6、IL-1β)的分泌。TSG能明显增加糖尿病大鼠心脏SIRT1和p AMPK蛋白表达。结论 TSG对糖尿病大鼠心肌具有保护作用,其机制可能与抑制心肌炎症因子和改善能量代谢有关。
[Abstract]:Objective to investigate the effects of stilbene glucoside (TSGG) on myocardial injury in diabetic rats and its effects on mammalian homologue 1 SIRT1 (silencing signaling factor 2) and adenosine phospho-activated protein kinase (AMPK) protein. Methods Type 2 diabetic rat models were established. Rats were killed after 16 weeks of administration, and blood glucose and blood lipids were measured by biochemical method. Liver function and creatine kinase CKH, lactate dehydrogenase (LDH) and free fatty acid in myocardial tissue were determined by enzyme linked immunosorbent assay (Elisa), cardiac troponin 鈪，

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