探讨溶血、乳糜血、自身抗体对HIV抗体初筛结果的影响

发布时间：2018-03-21 03:50

本文选题：溶血　切入点：乳糜血　出处：《东南大学学报(医学版)》2016年01期 　论文类型：期刊论文

【摘要】：目的:探讨溶血、乳糜血、自身抗体阳性标本对酶联免疫吸附测定人类免疫缺陷病毒(enzyme linked immunosorbent assay-human immunodeficiency virus,ELISA-HIV)抗体初筛结果的影响。方法:对80份对照血样本、80份不同程度的溶血、240份不同程度的乳糜血以及80份自身抗体阳性血样本同时用两种ELISA检测试剂检测HIV抗体,并对ELISA-HIV抗体待复检的血样本用蛋白质印迹法(Western blotting,WB)进行复检。结果:80份溶血标本和240份乳糜血样本中,分别有3份样本ELISA-HIV抗体结果待复检,造成ELISAHIV假阳性率分别为3.75%(3/80)和1.25%(3/240);80份自身抗体阳性血清样本检测后显示,4份样本HIV抗体结果待复检,它们分别来源于类风湿关节炎、系统性红斑狼疮、银屑病和特发性血小板减少性紫癜患者血清,自身抗体造成ELISA-HIV抗体假阳性率为5.00%(4/80)。结论:溶血、乳糜血、自身抗体对ELISA-HIV抗体初筛结果均有一定的影响,均可提高其假阳性率。
[Abstract]:Objective: to study hemolysis and chylous blood. The effect of autoantibody positive specimen on the primary screening results of linked immunosorbent assay-human immunodeficiency virusvirus-ELISA-HIVA antibody against human immunodeficiency virus by Elisa. Methods: 80 control blood samples were collected from 80 different degrees of hemolysis and 240 samples of chyme with different degrees of hemolysis. Blood and 80 samples of autoantibody positive blood were tested for HIV antibody with two kinds of ELISA detection reagents at the same time. Western blotting method was used to retest the blood samples of ELISA-HIV antibody. Results of 80 hemolytic samples and 240 chylous blood samples, 3 samples of ELISA-HIV antibody were retested. The false positive rates of ELISAHIV were 3.75 and 3.75 / 80, respectively) and 1.25% of 80 autoantibody positive serum samples were tested. After testing, the results of HIV antibodies of 4 samples were to be re-examined. They were derived from rheumatoid arthritis and systemic lupus erythematosus, respectively. The false positive rate of ELISA-HIV antibody caused by autoantibody in patients with psoriasis and idiopathic thrombocytopenic purpura was 5.00% / 80%. Conclusion: hemolysis, chylous blood, autoantibodies have certain influence on the results of primary screening of ELISA-HIV antibodies, and can improve the false positive rate of ELISA-HIV antibodies.
【作者单位】：南京市职业病防治院检验科;南京市职业病防治院皮肤科;
【分类号】：R512.91;R446.6

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