瞬时受体电位离子通道3在糖尿病肾病小鼠的表达及意义

发布时间：2018-03-21 03:52

本文选题：糖尿病肾病　切入点：瞬时受体电位离子通道　出处：《肾脏病与透析肾移植杂志》2017年02期 　论文类型：期刊论文

【摘要】：目的:研究瞬时受体电位离子通道3(TRPM3)在2型糖尿病肾病模型db/db小鼠肾组织的表达,以及高糖对人肾小管上皮细胞(HK2)和小鼠足细胞(MPC)TRPM3表达的影响。方法:(1)将20只雄性5周龄db/db小鼠随机分为两组,各10只。同窝出生的db/m小鼠作为正常对照组(n=20)。待db/db小鼠生长至10周龄和18周龄后取血、尿标本测定相关生化指标,留取肾组织。利用Western blot、实时荧光定量PCR(qRT-PCR)和免疫组织化学方法,检测正常对照组(db/m小鼠)及不同周龄(10周、18周)db/db小鼠肾组织中TRPM3的表达水平及分布情况。(2)体外培养HK2和条件永生化MPC,分别按以下处理分组:对照组(D-葡萄糖5.6 mmol/L);甘露醇组(D-葡萄糖5.6 mmol/L+甘露醇24.4 mmol/L);高糖组(D-葡萄糖30 mmol/L),培养24h、48h、72h收获细胞,用Western blot和qRT-PCR检测细胞中TRPM3的表达。结果:(1)与db/m小鼠相比,db/db小鼠肾组织肾小管和肾小球中TRPM3的表达增多,差异有统计学意义(P0.05);(2)与正常对照组相比,高糖组HK2和MPC中TRPM3的表达均增多,差异有统计学意义(P0.05);结论:TRPM3可表达于糖尿病肾病小鼠肾脏组织的肾小管和肾小球中,且高血糖可诱导TRPM3在HK2细胞和MPC细胞的高表达;TRPM3可能参与了糖尿病肾病的发生发展。
[Abstract]:Objective: to study the expression of transient receptor potential ion channel (3TRPM3) in the kidney of db/db mice with type 2 diabetic nephropathy. And the effects of high glucose on the expression of MPC-TRPM3 in human renal tubular epithelial cells (HK2) and mouse podocytes. Methods Twenty male 5-week-old db/db mice were randomly divided into two groups. Ten db/m mice born in the same nest were used as normal control group. Blood samples were collected from db/db mice after 10 and 18 weeks of age. Urine samples were measured and renal tissues were taken. Using Western blot, real-time fluorescence quantitative PCRQRT-PCRs and immunohistochemical method were used. The expression and distribution of TRPM3 in kidney tissue of normal control group (10 weeks, 18 weeks old) and control group (10 weeks and 18 weeks old) were detected. The HK2 and conditioned immortalized MPCs were cultured in vitro and divided into the following groups: control group: D- glucose 5.6 mmol / L; In mannitol group, D-glucose 5.6 mmol/L mannitol 24.4 mmol / L, and high glucose group 30 mmol / L, cultured for 24 h or 48 h for 72 h. Western blot and qRT-PCR were used to detect the expression of TRPM3 in the cells. Results compared with db/m mice, the expression of TRPM3 in renal tubules and glomeruli of db-db mice was higher than that of db/m mice, and the difference was statistically significant (P 0.05). The expression of TRPM3 in HK2 and MPC was increased in high glucose group, and the difference was statistically significant (P 0.05). Conclusion: TPM 3 can be expressed in renal tubules and glomeruli of diabetic nephropathy mice. Hyperglycemia can induce the high expression of TRPM3 in HK2 cells and MPC cells, which may be involved in the development of diabetic nephropathy.
【作者单位】：郑州大学第一附属医院肾脏内科;郑州大学肾脏病研究所;河南省高等学校临床医学重点学科开放实验室;
【分类号】：R587.2;R692.9

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