不同铝化合物对PC12细胞毒性作用比较

发布时间：2018-03-25 15:22

本文选题：铝　切入点：PC细胞　出处：《中国职业医学》2017年01期

【摘要】：目的研究4种不同种类的铝化合物对大鼠肾上腺髓质嗜铬瘤分化细胞株PC12细胞的毒性作用。方法取对数生长期PC12细胞,分别予麦芽酚铝(染毒剂量为0.0、0.1、0.2、0.4、0.8 mmol/L)、三氯化铝(染毒剂量为0.0、1.0、2.0、4.0、8.0 mmol/L)、柠檬酸铝(染毒剂量为0.0、1.0、2.0、4.0、8.0 mmol/L)和乳酸铝(染毒剂量为0.0、2.0、4.0、8.0、16.0 mmol/L)染毒24 h。采用CCK-8法检测细胞活力,以流式细胞术检测细胞凋亡率。结果随着4种铝化合物染毒剂量的增加,PC12细胞存活率均下降,细胞凋亡率均上升,均呈剂量-效应关系(P0.01)。对于麦芽酚铝、三氯化铝、柠檬酸铝和乳酸铝4种铝化合物,与对照组比较,导致PC12细胞活力出现有统计学意义下降的最低作用剂量(MEC)分别为0.2、2.0、2.0和4.0 mmol/L(P0.05),导致PC12细胞凋亡率出现有统计学意义升高的MEC分别为0.1、2.0、1.0和2.0 mmol/L(P0.05)。麦芽酚铝、三氯化铝、柠檬酸铝、乳酸铝对PC12细胞活力的24 h-半数抑制浓度分别为(0.45±0.01)、(4.02±0.39)、(5.37±0.88)和(6.31±0.58)mmol/L。结论 4种铝化合物均可引起PC12细胞的细胞活力下降和凋亡率升高,对PC12细胞均具有细胞毒性作用,且呈剂量-效应关系;但麦芽酚铝在较低剂量即对PC12细胞产生明显的剂量依赖性毒作用,以麦芽酚铝进行铝的离体神经毒性研究比其他铝化合物更具有优势。
[Abstract]:Objective to study the toxic effects of four kinds of aluminum compounds on rat adrenal medullary pheochromocytoma differentiation cell line PC12 cells. Aluminum-maltol (0. 01), aluminum trichloride (0. 0. 0 / L), aluminum trichloride (0. 0. 0 / L), aluminum citrate (0. 0. 0 ~ 2. 0 and 4. 08.0 mmol / L) and aluminum lactate (at a dose of 0. 02 / 0) and aluminium lactate (at a dose of 0. 02 / 0 ~ 4. 08.0g / L) for 24 h, respectively. Cell viability was measured by CCK-8 assay. Results with the increase of dose of four aluminum compounds, the survival rate of PC12 cells decreased, and the apoptosis rate increased in a dose-effect relationship (P 0.01). For aluminum-maltol, aluminum trichloride, aluminum trichloride, and aluminum-trichloride were detected by flow cytometry. Aluminum citrate and aluminum lactate were compared with the control group. The lowest dosages of PC12 cell viability were 0.2mmol-2.0 and 4.0 mmol 路L ~ (-1) P _ (0.05), respectively. The MEC of PC12 cell apoptosis was 0.1mmol / L ~ (1.0) and 2.0 mmol / L ~ (-1) (P _ (0.05)), respectively, and that of maltophenol aluminum, aluminum trichloride, aluminum citrate was higher than that of the control group, and that of PC12 cell apoptosis was 0.1mmol / L ~ (-1) and 2.0 mmol / L ~ (-1) (P _ (0.05)), respectively. The 24 h- half inhibitory concentrations of aluminum lactate on PC12 cell viability were 0.45 卤0.01, 4.02 卤0.39, 5.37 卤0.88) and 6.31 卤0.58 mmol 路L ~ (-1), respectively. Conclusion the four aluminum compounds can induce the decrease of cell viability and increase of apoptosis in PC12 cells, and all of them have cytotoxic effects on PC12 cells. In a dose-dependent manner, altol had a dose-dependent toxicity on PC12 cells at a lower dose, and the in vitro neurotoxicity of aluminum-maltol was superior to that of other aluminum compounds.
【作者单位】：山西医科大学公共卫生学院;
【基金】：国家自然科学基金(81430078,81372968)
【分类号】：R135.1

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