黄岑苷对实验性自身免疫性脑脊髓炎小鼠的影响

发布时间：2018-03-25 17:35

本文选题：黄岑苷　切入点：实验性自身免疫脑脊髓炎　出处：《中国比较医学杂志》2017年03期

【摘要】：目的观察不同浓度黄岑苷(baicalin)对实验性自身免疫脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)小鼠模型的作用,并研究其初步机制。方法建立实验性自身免疫性脊髓炎小鼠模型,免疫后第3天给予高低剂量黄岑苷灌胃,每天1次,共20 d。对小鼠活动进行神经功能评分,TUNEL染色检测脊髓组织细胞凋亡情况,ATP水平检测试剂盒检测脊髓组织ATP含量水平,免疫印迹法(Western blot)检测Bax、Bcl-2、cleaved cas-9和cleaved cas-3蛋白表达水平。结果黄岑苷能够提高实验性自身免疫脑脊髓炎小鼠神经功能,延后发病时间;黄岑苷干预后,Tunel染色结果显示脊髓组织凋亡细胞数下降,ATP水平下降,免疫印迹法结果显示Bcl-2的蛋白表达显著上升,Bax、cleaved cas-9和cleaved cas-3的蛋白表达显著下降。结论黄岑苷可通过抑制线粒体内源性凋亡途径抑制细胞凋亡,保护线粒体,提高实验性自身免疫脑脊髓炎小鼠神经功能,为预防疾病提供了实验理论依据。
[Abstract]:Objective to observe the effects of different concentrations of baicalin on experimental autoimmune encephalomytis (EAE) mouse model and to study its mechanism. Methods the experimental autoimmune myelitis model was established in mice. On the third day after immunization, the mice were administrated with high and low doses of Huangtsyn once a day for 20 days. The neuronal function score and Tunel staining were used to detect the apoptosis of spinal cord cells and the level of ATP in spinal cord tissue. Western blotting was used to detect the expression of cas-9 and cleaved cas-3 in Baxia Bcl-2cleaved. Results the results showed that baicin could improve the neural function of mice with experimental autoimmune encephalomyelitis and delay the onset time. The results of Tunel staining showed that the number of apoptotic cells in spinal cord decreased and ATP level decreased. The results of Western blotting showed that the protein expression of Bcl-2 increased significantly, and the protein expression of cleaved cas-3 and cas-9 decreased significantly. Conclusion the results suggest that bax cleave cas-9 and cleaved cas-3 can inhibit apoptosis and protect mitochondria by inhibiting the endogenous apoptosis pathway of mitochondria. To improve the neural function of mice with experimental autoimmune encephalomyelitis and to provide experimental theoretical basis for the prevention of disease.
【作者单位】：南阳市中心医院神经内科;
【分类号】：R744.51

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