HIV感染者调节性T细胞表面B、T淋巴细胞衰减因子的表达研究

发布时间：2018-03-27 17:08

本文选题：HIV　切入点：Treg　出处：《中国免疫学杂志》2017年07期

【摘要】：目的:对HIV感染者调节性T细胞(Regulatory T cell,Treg)上B、T淋巴细胞衰减因子(B and T lymphocyte attenuator,BTLA)的表达水平进行检测,并探讨其在HIV感染进程中的作用。方法:选取24例感染在一年之内的HIV早期感染者(Early HIV infected patients,EHI组)、14例感染超过一年的HIV慢性感染者(CD4+T计数200 cells/μl,HIV组)、6例AIDS患者(CD4+T计数200 cells/μl,AIDS组)和9例健康人作为对照,应用流式细胞仪检测不同时期感染者及健康对照者Treg细胞BTLA的表达水平,分析其与疾病进展及免疫活化的相关性。结果:随着HIV疾病进展,EHI组、HIV组及AIDS组Treg细胞BTLA表达水平依次升高,其中HIV组与AIDS组Treg细胞BTLA表达水平显著高于EHI组(P0.05及P0.01),AIDS组Treg细胞BTLA的表达水平高于健康对照(P0.05);Treg细胞BTLA表达水平与CD4~+T淋巴细胞计数呈负相关(P0.001),与病毒载量呈正相关(P0.01);Treg细胞BTLA表达水平与活化CD4~+CD38~+T淋巴细胞及CD4~+HLA-DR~+T淋巴细胞呈正相关(P0.001,P0.001)。结论:HIV感染者Treg细胞BTLA表达升高,与疾病进展显著相关,提示其可能通过加强Treg细胞的抑制功能加速疾病进展,并为未来HIV感染的干预提供信息。
[Abstract]:Objective: to detect the expression of B and T lymphocyte attenuator (BTLAs) on regulatory T cells of HIV infected patients. To explore its role in the process of HIV infection. Methods: the early HIV infected patientsEHI group of 24 patients with early HIV infection within one year were selected. There were 14 patients with chronic HIV infection who had been infected with HIV for more than a year. The CD4 T count of 6 AIDS patients with HIV infection was 200 cells/ 渭 l. #number0# cells/ 渭 l AIDS-group and 9 healthy persons were counted as controls. Flow cytometry was used to detect the expression of BTLA in Treg cells of patients with different stages of infection and healthy controls. Results: with the progression of HIV disease, the BTLA expression level of Treg cells in AIDS group and AIDS group increased in turn. The expression of BTLA in Treg cells in HIV group and AIDS group was significantly higher than that in EHI group (P0.05) and P0.01 HIV group. The BTLA expression level of Treg cell in HIV group was significantly higher than that in healthy control group (P 0.05). There was a negative correlation between BTLA expression level and CD4 ~ T lymphocyte count in HIV group and AIDS group, and a positive correlation between BTLA expression level and viral load. There was a positive correlation between the expression of BTLA and activated CD4 ~ CD38 ~ T lymphocytes and CD4 ~ HLA-DRT lymphocytes. Conclusion the expression of BTLA in Treg cells was increased. It may accelerate the disease progression by strengthening the inhibitory function of Treg cells and provide information for the intervention of HIV infection in the future.
【作者单位】：中国医科大学附属第一医院检验科国家卫生计生委艾滋病免疫学重点实验室;
【基金】：国家自然科学基金(81371884) 基础临床紧密结合平台项目(医大发字[2013]5号)项目资助
【分类号】：R512.91

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