联合使用固力康和特立帕肽对去卵巢大鼠股骨干骺端骨量、骨强度的影响

发布时间：2018-04-13 13:36

本文选题：去势大鼠 + 骨质疏松症　；参考：《中国骨质疏松杂志》2017年02期

【摘要】：目的探索联合使用特立帕肽(PTH)和固力康(KKI)对去势大鼠骨质疏松的防治作用。方法 50只健康雌性SD大鼠随机行假手术(Sham,n=10)和切除双侧卵巢(OVX,n=40)手术后,OVX大鼠随机的分成4组:OVX组、KKI组、PTH组、PTH+KKI组。术后第一天开始给予药物治疗,KKI组:固力康灌胃(剂量6 mg/kg,每天1次),PTH组:PTH皮下注射(剂量60μg/kg,每周3次),PTH+KKI组:固力康灌胃和特立帕肽皮下注射,剂量和用药频率和KKI组、PTH组相同,直至术后12 w为止,12 w时所有大鼠处死取股骨行Micro-CT、HE染色切片及骨生物力学检测。结果结果表明KKI组、PTH组、PTH+KKI组和OVX组相比,股骨干骺端都有较高的BMD、BV/TV、Tb.Th、Tb.N、Conn.D、最大的载荷、刚度和较低的Tb.Sp,其中PTH+KKI组大鼠胫骨有最高的BMD、BV/TV、Tb.Th、Tb.N、Conn.D、最大的载荷、刚度和最低Tb.Sp。KKI和PTH单独使用的效果明显低于他们联合使用对去卵巢大鼠骨质疏松的防治作用。结论联合使用固力康和特立帕肽对去势大鼠骨质疏松的防治有叠加作用。
[Abstract]:Objective to explore the preventive and therapeutic effects of combined use of maopa peptide (PTH) and KKI on osteoporosis in ovariectomized rats.Methods Fifty healthy female Sprague-Dawley rats were randomly divided into 4 groups after sham-operation and ovariectomized OVX KKI.On the first day after operation, the KKI group was given drug therapy: Gulikang group was given intragastric perfusion (6 mg / kg, once a day) (60 渭 g / kg) (60 渭 g / kg, 3 times a week): Gulikang group was given intragastric administration and maopapeptide was subcutaneously injected.The dosage and frequency of administration were the same as those in KKI group, and all the rats were killed until 12 weeks after operation. Micro-CTHE staining sections and bone biomechanical examination were performed.The effects of stiffness and minimum Tb.Sp.KKI and PTH alone on osteoporosis in ovariectomized rats were significantly lower than those in combination.Conclusion combined use of Gulikang and maopapeptide has superposition effect on the prevention and treatment of osteoporosis in ovariectomized rats.
【作者单位】：湖北省荆门市第一人民医院创伤手足外科;
【分类号】：R580

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