硫化氢对高脂饮食诱导的肥胖小鼠脂肪代谢的调控作用

发布时间：2018-04-16 23:02

本文选题：硫化氢 + 饮食致胖　；参考：《复旦学报(医学版)》2016年06期

【摘要】：目的探讨给予外源性硫化氢(H2S)对肥胖小鼠脂肪代谢的调控作用。方法将小鼠分为正常对照组、模型组和治疗组。正常对照组(n=10):给予正常小鼠饲料喂养;模型组(n=10):给予高脂饲料喂养12周建立饮食致胖(diet-induced obesity,DIO)模型;治疗组(n=15):同样建立成DIO小鼠并随机分3小组,分别给予25、50和100μmol·kg-1·d-1 NaHS治疗6周,期间继续给予高脂饲料。给药期间动态监测各组小鼠的体质量变化。治疗期满处死小鼠,检测附睾脂肪以及肾周脂肪重量,用自动生化仪和ELISA法分别检测小鼠血清中的游离脂肪酸(non-esterified fatty acid,NEFA)和抵抗素(resistin)脂肪因子水平。结果与正常对照组比较,DIO模型组小鼠造模成功,体质量、附睾脂肪重量与肾周脂肪重量均有明显升高,但小鼠血清中的NEFA和resistin含量没有明显变化;与模型组相比,治疗各组小鼠附睾脂肪重量与肾周脂肪重量均有明显的回落(P0.05),血清中的NEFA水平也明显降低(P0.05),resistin含量没有显著变化。结论 H2S可以显著缓解DIO小鼠脂肪的堆积,降低治疗组血清中NEFA的水平,但对resistin脂肪因子无明显调控作用。
[Abstract]:Objective to investigate the effects of exogenous hydrogen sulfide (H 2 S) on fat metabolism in obese mice.Methods mice were divided into normal control group, model group and treatment group.The normal control group was fed with normal diet, the model group was fed with high fat diet (12 Zhou Jianli diet), the treatment group was fed with 12 diet, and the treatment group was established into DIO mice and randomly divided into 3 groups, which were treated with 2550 and 100 渭 mol kg-1 d-1 NaHS for 6 weeks, respectively.During the period continue to give high fat feed.The changes of body mass in each group were monitored dynamically during administration.The epididymal fat and perirenal fat weight were measured at the end of the treatment. The levels of serum free fatty acid, non-esterified fatty acidinine (NEFAA) and resistin resistinin (resistin) in serum of mice were detected by automatic biochemical instrument and ELISA method, respectively.Results compared with the normal control group, the mice in the DIO model group were successfully made, the body weight, epididymal fat weight and perirenal fat weight were significantly increased, but the contents of NEFA and resistin in the serum of the mice were not significantly changed, and those in the model group were significantly higher than those in the model group.The weight of epididymal fat and perirenal fat were significantly decreased in each group, and the level of NEFA in serum did not change significantly.Conclusion H2S can significantly alleviate the fat accumulation in DIO mice and decrease the level of serum NEFA in the treatment group, but it has no effect on the regulation of resistin fat factor.
【作者单位】：复旦大学基础医学院生理与病理生理学系;上海市小分子活性物质重点实验室;
【基金】：国家自然科学基金(81230003,31471088)~~
【分类号】：R589.2

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