A2M治疗Ⅱ型胶原诱导性踝关节炎的疗效评价

发布时间：2018-04-28 14:55

本文选题：踝关节 + 类风湿性关节炎　；参考：《山西医科大学》2017年硕士论文

【摘要】：目的:1、CIA小鼠的踝关节腔中补充额外A2M,能否起到抑制炎症、保护关节软骨及骨的作用;2、较高浓度的A2M是否有更好的抑制炎症及保护关节作用;3、FMT能否作为一种敏感的动态活体生物成像监测软骨及骨损伤的手段。方法:选用健康2月龄雌性DBA/1小鼠60只,随机平均分为4组,第1、2、3组进行CIA造模,第4组作为阴性对照。于第33d时所有CIA小鼠踝关节均出现发红及肿胀,于第35d时X光检测踝关节,鉴别其炎症及关节破坏情况。于第36d、42d、49d时,前3组右侧踝关节腔内分别注射浓度为0.04mg/ml、0.08mg/ml和0.12mg/ml的A2M溶液10ul。于第33d-57d,每4天1次,进行小鼠尾静脉注射ProSense FAST和MMPSense FAST荧光标记物,并于每次注射标记物后24h使用FMT对模型鼠进行活体炎症检测。于第57d,FMT及X光检测后处死小鼠,取踝关节进行PCR、番红O染色以及免疫组织化学检测。结果:于第33d时,所有CIA小鼠踝关节均出现发红与肿胀,模型建立成功,第35d X光显示,踝关节局部存在炎症反应但并无骨质破坏。治疗期间FMT结果显示,A2M治疗组炎症反应明显低于阳性对照组,有显著统计学意义;且A2M浓度越高,炎症抑制越明显;另X线、PCR、番红O染色及免疫组织化学结果均与FMT结果相一致,表明关节炎症反应减轻,同时治疗组的A2M在一定程度上可以抑制滑膜增生、保护关节软骨减轻骨质破坏。结论:早期使用A2M治疗可以有效抑制CIA动物模型的炎症反应,延缓病变进展程度,且其浓度越高,效果越好;同时FMT可以成为一种有效的活体检测方法,实时监控并预测病情的发展程度。
[Abstract]:Objective to investigate whether the supplementation of extra A2M into the ankle cavity of the CIA mice can inhibit inflammation. The protective effect of articular cartilage and bone is 2%. Whether the higher concentration of A2M can inhibit inflammation and protect joints can FMT be used as a sensitive dynamic in vivo imaging to monitor cartilage and bone injury. Methods: 60 healthy 2 month old female DBA/1 mice were randomly divided into 4 groups. Group 1 and group 2 were used to model CIA, and group 4 was used as negative control. At the 33rd day, all the CIA mice showed redness and swelling of the ankle joint. At the 35th day, the ankle joint was detected by X-ray to distinguish the inflammation and joint destruction. On the 48th day of 36d, the first three groups were injected with 0.04 mg / ml 0.08 mg / ml A2M solution and 10 ululums respectively. ProSense FAST and MMPSense FAST fluorescent markers were injected into caudal vein of mice every 4 days at 33d-57d and FMT was used 24 hours after each injection to detect in vivo inflammation in model mice. The mice were killed after FMT and X ray examination on the 57th day, and the ankle joints were detected by PCR, Phanerin O staining and immunohistochemistry. Results: on the 33th day, all the CIA mice showed redness and swelling of the ankle joint, and the model was established successfully. On the 35th day, the local inflammation of the ankle joint was found, but no bone destruction was found. FMT results showed that the inflammatory response of A2M treatment group was significantly lower than that of the positive control group, and the higher the A2m concentration, the more obvious the inflammatory inhibition. The results of FMT showed that the reaction of arthritis was alleviated, and A2m in the treatment group could inhibit synovial hyperplasia and protect articular cartilage from bone destruction to some extent. Conclusion: early treatment with A2M can effectively inhibit the inflammatory response of CIA animal model and delay the progress of the disease, and the higher the concentration of A2m, the better the effect, and FMT can be an effective method for in vivo detection. Monitor and predict the development of the disease in real time.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R593.22

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