亚砷酸钠中毒对大鼠海马组织神经元及肾功能的影响研究

发布时间：2018-04-28 18:47

本文选题：砷中毒 + CA区　；参考：《中国全科医学》2017年26期

【摘要】：目的观察亚砷酸钠(NaAsO_2)中毒对成年SD大鼠海马组织CA3区的神经元及肾脏形态学的影响。方法于2016年2—6月,48只12周龄雄性SPF级SD大鼠随机分为对照组及低、中、高染毒组,每组各12只。将NaAsO_2用蒸馏水分别配制成5、10、20 mg/kg溶液分别供低、中、高染毒组大鼠自由饮用,对照组可自由饮用蒸馏水。建造染砷模型12周后,取4组大鼠脑组织海马部分和肾组织,HE染色光镜观察海马组织神经元与肾脏形态学改变;同时测定海马组织与肾组织中超氧化物歧化酶(SOD)活力、还原型谷胱甘肽(GSH)及丙二醛(MDA)含量。结果不同剂量染毒组海马组织神经元与对照组比较,神经元胞体形态欠规则,排列稀疏且数量减少,镜下可见大量脱落及坏死的神经元。不同剂量染毒组的肾组织较对照组出现病理性改变,例如变性、坏死、组织出现水肿及炎性细胞浸润等。4组大鼠海马组织及肾组织SOD活力、GSH和MDA含量比较,差异均有统计学意义(P0.05);其中低、中、高染毒组海马组织及肾组织SOD活力、GSH含量均低于对照组,MDA含量均高于对照组;中、高染毒组海马组织及肾组织SOD活力、GSH含量均低于低染毒组,MDA含量均高于低染毒组;高染毒组海马组织及肾组织SOD活力、GSH含量均低于中染毒组,MDA含量均高于中染毒组(P0.05)。结论 NaAsO_2中毒可使大鼠海马组织神经元形态结构及肾组织结构损伤,同时提示氧化应激可能参与NaAsO_2致大鼠海马组织及肾组织的毒性作用。
[Abstract]:Objective to observe the effects of sodium arsenite (NaAsO _ 2) poisoning on neurons and renal morphology in the CA3 region of hippocampus of adult SD rats. Methods from February to June 2016, 48 12-week-old male SD rats of SPF grade were randomly divided into control group and low, medium and high exposure groups with 12 rats in each group. NaAsO_2 was prepared with distilled water into 5 ~ 10 ~ 10 ~ 20 mg/kg solution for free drinking of rats in low, medium and high exposure groups, while in the control group, distilled water was free to drink. The morphological changes of neurons and kidneys in hippocampus and kidney were observed by HE staining and the activity of superoxide dismutase (SOD) in hippocampal and renal tissues were measured by HE staining after 12 weeks of arsenic exposure. Reduced glutathione (GSH) and malondialdehyde (MDA). Results compared with the control group, the hippocampal neurons in the different dose groups were irregular in shape, sparse in arrangement and reduced in number. A large number of neurons were found to be shedding and necrotizing under microscope. The contents of SOD and MDA in hippocampal and renal tissues of rats in different dose groups were compared with those in control group, such as degeneration, necrosis, edema and inflammatory cell infiltration. The content of SOD in hippocampus and kidney in the low, middle and high exposure groups were lower than those in the control group, and the content of SOD in the control group was higher than that in the control group. The content of SOD in hippocampus and kidney in high exposure group was lower than that in low exposure group, and the content of SOD activity in high exposure group was lower than that in moderate exposure group. Conclusion NaAsO_2 poisoning can damage hippocampal neurons and renal tissues in rats, and it is suggested that oxidative stress may be involved in the toxic effects of NaAsO_2 on hippocampal and renal tissues in rats.
【作者单位】：石河子大学医学院人体解剖与组织胚胎学教研室;石河子大学第一附属医院肝胆外科;衢州职业技术学院医学院;
【基金】：国家自然科学基金资助项目(81360410)
【分类号】：R595.2

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