培哚普利通过调节Akt-FoxO1通路保护糖尿病性心肌病大鼠心功能损伤

发布时间：2018-05-04 10:31

本文选题：糖尿病性心肌病 + 培哚普利　；参考：《山东大学学报(医学版)》2017年10期

【摘要】：目的探讨培哚普利对糖尿病性心肌病(DCM)大鼠心功能损伤的影响及其潜在的作用机制。方法 40只8周龄(100~120 g)雄性Sprague-Daw ley大鼠随机分为对照组(n=10)、DCM组(n=15)和DCM+培哚普利组(n=15)。对照组给予普通饮食饲养,其他两组制备糖尿病性心肌病大鼠模型。模型建立成功后,DCM组给予高脂饮食饲养和生理盐水灌胃,DCM+培哚普利组给予高脂饮食饲养和培哚普利灌胃4 mg/(kg·d)。观察培哚普利对糖尿病性心肌病大鼠心功能、心肌细胞凋亡和心肌纤维化的影响,以及其对Akt-Fox O1信号通路的影响。结果 DCM组较对照组心功能降低,心肌细胞凋亡和心肌纤维化程度增加,Akt的磷酸化水平降低,Fox O1的蛋白表达升高,两组差异有统计学意义(P0.05);DCM+培哚普利组较DCM组心功能损伤减轻,并影响AktFox O1的表达水平,两组差异有统计学意义(P0.05)。结论培哚普利通过调节Akt-Fox O1信号通路抑制DCM大鼠的心肌细胞凋亡和心肌纤维化,保护DCM大鼠的心功能损伤。
[Abstract]:Objective to investigate the effect of perindopril on cardiac function injury in diabetic cardiomyopathy (DM) rats and its possible mechanism. Methods Forty 8-week-old male Sprague-Daw ley rats were randomly divided into control group (n = 10) and DCM perindopril group (n = 15). The control group was fed with general diet and the other two groups were given diabetic cardiomyopathy model. After the successful establishment of the model, the DCM group was fed with high-fat diet and the saline perindopril group was fed with high-fat diet and perindopril for 4 mg/(kg. To observe the effects of perindopril on cardiac function, cardiomyocyte apoptosis and myocardial fibrosis in diabetic cardiomyopathy rats, and the effects of perindopril on Akt-Fox O 1 signaling pathway. Results compared with the control group, the cardiac function of DCM group was lower than that of the control group, and the level of myocardial apoptosis and myocardial fibrosis was increased. The expression of DCM O1 protein was increased. There was a significant difference between the two groups. Compared with the DCM group, the cardiac function damage in the DCM group was less than that in the DCM group. The expression level of AktFox O 1 was significantly affected by the difference between the two groups (P 0.05). Conclusion Perindopril inhibits cardiomyocyte apoptosis and myocardial fibrosis in DCM rats by regulating the Akt-Fox O 1 signaling pathway and protects the heart function of DCM rats.
【作者单位】：山东大学附属济南市中心医院心内科;
【分类号】：R542.2;R587.2

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