类风湿关节炎复合高血压疾病模型的建立及评价

发布时间：2018-06-02 05:23

本文选题：类风湿关节炎复合高血压 + 佐剂性关节炎　；参考：《中国比较医学杂志》2017年08期

【摘要】：目的建立类风湿关节炎复合高血压疾病(arthritis-hypertension disease,AHD)大鼠模型,并对该模型的特点进行评价。方法实施外科手术用0.25 mm银夹缩窄大鼠左肾动脉建立高血压(hypertension,HT)模型,同时于大鼠左后足跖注射0.1 m L完全弗氏佐剂致炎建立AHD模型。无创血压测量分析系统测量尾动脉压。足爪容积测量仪测量大鼠非致炎侧关节肿胀度,并分析关节炎指数与炎症发生率。第35天处死大鼠,收集胸主动脉、踝关节及脾脏组织HE染色并进行病理学检查。结果 AHD模型大鼠关节明显肿胀,关节腔大量滑膜细胞增生、炎症细胞浸润、血管翳形成,脾脏生发中心数量增加、大量淋巴细胞浸润、白髓弥漫性增生、红髓浸润。关节炎指数、炎症发生率以及关节、脾脏组织病理评分较佐剂性关节炎(adjuvant arthritis,AA)大鼠明显升高;同时,AHD模型大鼠血压显著升高,胸主动脉中膜厚度、横截面积明显增加,管腔直径明显降低,且其血压值和血管损伤程度较HT大鼠明显升高或加重。结论用完全弗氏佐剂足跖皮内注射法合并外科手术缩窄左肾动脉法可成功建立大鼠AHD模型。
[Abstract]:Objective to establish a rat model of arthritis-hypertension disease (AHD) combined with rheumatoid arthritis (RA) and to evaluate the characteristics of the model. Methods the model of hypertensionHT was established by using 0.25 mm silver clip constricted left renal artery in rats. AHD model was established by injecting 0.1 mL complete Freund's adjuvant into the left hind plantar of rats. The non-invasive blood pressure measurement system was used to measure the caudal artery pressure. The degree of non-inflammatory joint swelling was measured by paw volumetric instrument, and the arthritis index and the incidence of inflammation were analyzed. On the 35th day, the rats were killed, the thoracic aorta, ankle and spleen were stained with HE and examined by pathology. Results the joints of AHD rats were obviously swollen, a large number of synovial cells proliferated, inflammatory cells infiltrated, pannus formed, the number of splenic germinal centers increased, a large number of lymphocytes infiltrated, diffuse proliferation of white pulp, and infiltration of red pulp. The index of arthritis, the incidence of inflammation, the pathological score of joint and spleen tissue were significantly higher than those of adjuvant arthritis (AAA) rats, and the blood pressure, the thickness of thoracic aorta and the cross sectional area of thoracic aorta were significantly increased in rats with AHD. The diameter of the lumen was significantly decreased, and the blood pressure and vascular injury degree were significantly increased or aggravated than that of HT rats. Conclusion the rat AHD model can be successfully established by complete Freund's adjuvant intraplantar injection combined with surgical constriction of left renal artery.
【作者单位】：安徽医科大学临床药理研究所抗炎免疫药物教育部重点实验室安徽省抗炎免疫药物协同创新中心;
【基金】：国家自然科学基金(81330081,81302784,81673444) 安徽省自然科学基金(1508085MH182) 安徽医科大学博士科研资助基金(XJ201534)
【分类号】：R-332;R544.1;R593.22

【相似文献】