老龄慢性酒精中毒大鼠模型大脑中动脉力学特性

发布时间：2018-06-15 13:59

本文选题：慢性酒精中毒 + 力学特性　；参考：《中国老年学杂志》2017年24期

【摘要】：目的探讨正常与老龄慢性酒精中毒大鼠大脑中动脉的力学特性。方法建立老龄慢性酒精中毒大鼠模型,随机分为模型组与对照组,建模第55天起对各组大鼠进行Morris水迷宫实验,取两组大鼠大脑中动脉进行拉伸实验,将两组大鼠断头后快速取脑,漂洗,将额区皮质分离后以生理盐水为介质,脑组织与生理盐水重量体积比为9∶1,制成10%脑组织匀浆液,离心后取上清液0.2 ml加入试管,测量丙二醛(MDA)、超氧化物歧化酶(SOD)和金属硫蛋白(MT)含量。结果模型组各时间点逃避潜伏期值显著大于对照组(P0.05)。模型组SOD和MT含量显著小于对照组,MDA含量显著大于对照组(均P0.05)。模型组大鼠大脑中动脉弹性限度应变、弹性限度应力、最大应力、最大应变均显著小于对照组,弹性模量显著大于对照组(均P0.05)。结论老龄慢性酒精中毒大鼠模型大脑中动脉力学特性发生了改变。
[Abstract]:Objective to investigate the mechanical properties of middle cerebral artery in normal and aged rats with chronic alcoholism. Methods the aged rats with chronic alcoholism were randomly divided into two groups: the model group and the control group. Morris water maze test was performed on each group from the 55th day of the model, and the middle cerebral artery of the two groups was taken for stretching test. After decapitation, the brain of the two groups was quickly removed and rinsed. The cortex of the frontal area was separated with normal saline as the medium. The brain tissue was made into 10% brain homogenate with the weight volume ratio of 9: 1. After centrifugation, the supernatant 0.2 ml was added to the test tube. The contents of malondialdehyde (MDA), superoxide dismutase (SOD) and metallothionein (MTX) were measured. Results the escape latency at each time point in the model group was significantly higher than that in the control group (P 0.05). The content of SOD and MT in the model group was significantly lower than that in the control group (P 0.05). The elastic limit strain, elastic limit stress and maximum strain of the middle cerebral artery in the model group were significantly lower than those in the control group, and the elastic modulus of the model group was significantly higher than that of the control group (all P 0.05). Conclusion the mechanical properties of middle cerebral artery were changed in aged rats with chronic alcoholism.
【作者单位】：长春市第六医院精神科;吉林大学中日联谊医院超声科;吉林大学中日联谊医院疼痛科;长春市第六医院司法鉴定科;
【基金】：吉林省科技发展计划资助项目(No.20110492)
【分类号】：R-332;R595.6

【相似文献】