microRNA-181c在重症肌无力患者外周血单个核细胞中的表达及其意义

发布时间：2018-06-16 14:00

本文选题：重症肌无力 + 人外周血单个核细胞　；参考：《中风与神经疾病杂志》2017年10期

【摘要】：目的探讨microRNA-181c(miR-181c)在重症肌无力(myasthenia gravis,MG)患者中的表达水平及其临床意义。方法收集2015年6月-2017年6月收治的22例MG患者和20例健康人的外周静脉血样,提取外周血单个核细胞(peripheral blood mononuclear cell,PBMC)中总RNA,采用实时定量PCR(quantitative real-time PCR,qRT-PCR)技术检测PBMC中miR-181c的相对表达水平,并分析miR-181c表达水平与患者的MG定量评分体系(quantitative myasthenia gravis score,QMG)的相关性。结果 MG患者PBMC中miR-181c的相对表达水平(0.303±0.150)较对照组(1.000±0.262)显著降低(P0.01),其中全身型患者(0.182±0.056)低于眼肌型患者(0.404±0.126)(P0.05),同时miR-181c表达水平与QMG呈负相关(R2=-0.359,P0.05)。结论 MG患者PBMC中miR-181c表达水平的降低可能与病情严重程度有关。
[Abstract]:Objective to investigate the expression and clinical significance of microRNA-181 cmiR-181c in myasthenia gravis (MGG) patients with myasthenia gravis. Methods Peripheral venous blood samples were collected from 22 MG patients and 20 healthy controls from June 2015 to June 2017. Total RNAs from peripheral blood mononuclear cells (PBMCs) were extracted. The relative expression of miR-181c in peripheral blood mononuclear cells (PBMCs) was detected by real-time quantitative real-time qRT-PCR. The correlation between miR-181c expression and quantitative myasthenia gravis score system was analyzed. Results the relative expression level of miR-181c in PBMC of MG patients (0.303 卤0.150) was significantly lower than that of control group (1.000 卤0.262), and the expression of miR-181c in patients with systemic type was 0.182 卤0.056) lower than that in patients with ocular muscle type (0.404 卤0.126), and the expression level of miR-181c was negatively correlated with QMG. Conclusion the decrease of miR-181c expression in PBMC of MG patients may be related to the severity of the disease.
【作者单位】：南京医科大学附属淮安第一医院急诊科;徐州医科大学附属医院神经内科;
【分类号】：R746.1

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