胰升血糖素通过环磷鸟嘌呤核苷信号通路调节MIN6细胞胰岛素分泌的作用机制研究

发布时间：2018-07-04 15:26

本文选题：胰升血糖素 + 葡萄糖　；参考：《中国糖尿病杂志》2016年08期

【摘要】：目的探讨不同浓度的胰升血糖素(Glg)和葡萄糖(Glu)对胰岛β细胞株MIN6中胰岛素(Ins)分泌功能及细胞内环磷鸟嘌呤核苷(cGMP)信号通路的影响。方法用不同浓度Glu(0、2.8、16.7 mmol/L)及Glg(0、100、500、1000 ng/L)处理MIN6细胞。ELISA测定细胞内cGMP含量,同时测定细胞上清液中Ins的含量。结果单纯Glg处理组中,1000 ng/L组cGMP含量较500 ng/L组、100 ng/L组低(P0.05)。2.8 mmol/L Glu加不同浓度Glg处理组中,1000 ng/L Glg组cGMP含量较100 ng/L Glg组及0 ng/L Glg组低;500 ng/L Glg组较0 ng/L Glg组低(P0.05)。16.7 mmol/L Glu加不同浓度Glg处理组中,1000 ng/L Glg组cGMP含量较其他组低;500 ng/L Glg组较100 ng/L Glg组及0 ng/L Glg组低(P0.05)。单纯Glg处理组中,随Glg浓度升高,Ins分泌量升高(P0.05)。2.8 mmol/L Glu加不同浓度Glg处理组中,1000 ng/L Glg组Ins较100 ng/L Glg组及0 ng/L Glg组高;500 ng/L Glg组Ins较0 ng/L Glg组高(P0.05)。16.7 mmol/L Glu加不同浓度Glg处理组中,随Glg浓度升高,Ins分泌量升高(P0.05)。结论 Glg可能通过浓度梯度的方式下调MIN6细胞内cGMP的产生从而促进MIN6细胞Ins分泌,且这种作用有Glu依赖性。
[Abstract]:Objective to investigate the effects of different concentrations of glucagon (Glg) and glucose (Glu) on insulin (ins) secretion and intracellular cyclic guanosine (cGMP) signaling pathway in human islet 尾 cell line MIN6. Methods MIN6 cells were treated with different concentrations of Glu (0 2. 810. 7 mmol / L) and Glg (0 100 ~ 100 ~ 500 ~ 1 000 ng / L). The content of cGMP was measured by Elisa and the content of ins in supernatant of cells was also measured by enzyme-linked immunosorbent assay (Elisa). Results the cGMP content in 1000 ng / L group was lower than that in 100 ng / L group of 500 ng / L group (P0.05). 2.8 mmol / L Glu plus different concentrations of Glg group. The cGMP content in 1000 ng / L Glg group was lower than that in 100 ng / L Glg group and 0 ngL Glg group (P0.05). 16.7 mmol / L Glu content was significantly lower than that in 0 ng / L Glg group (P0.05). The content of cGMP in 1000ng / L Glg group was lower than that in other groups (P 0.05). The content of cGMP was lower in 500ng / L Glg group than that in 100ng / L Glg group and 0 ng / L Glg group (P0.05). The secretion of Ins increased with the increase of Glg concentration (P0.05) .2.8 mmol / L Glu + different concentration Glg treatment group. The ins of 1000 ng / L Glg group was higher than that of 100 ng / L Glg group and 0 ng / L Glg group, and the ins of 0 ng / L Glg group was higher than that of 0 ng / L Glg group (P0.05). 16.7 mmol / L Glu + different concentration Glg treatment group. The secretion of Ins increased with the increase of Glg concentration (P0.05). Conclusion Glg may down-regulate the production of cGMP in MIN6 cells and promote the secretion of Ins in MIN6 cells by concentration gradient, and this effect is Glu dependent.
【作者单位】：新疆石河子大学医学院;新疆石河子大学医学院第一附属医院内分泌代谢科;
【基金】：兵团中青年科技创新领军人才计划(2015BC001) 兵团科技支疆计划项目(2014AB049) 兵团博士资金专项(2012BB019)
【分类号】：R587.1

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