金黄色葡萄球菌显著增加系统性红斑狼疮患者中性粒细胞NETs产生

发布时间：2018-07-05 11:45

本文选题：系统性红斑狼疮 + 金黄色葡萄球菌　；参考：《免疫学杂志》2017年06期

【摘要】：目的探讨金黄色葡萄球菌对系统性红斑狼疮(SLE)中性粒细胞胞外诱捕网(NETs)产生的影响及意义。方法收集SLE稳定者22例、活动者20例和健康对照18例,分离中性粒细胞并加金黄色葡萄球菌共培养,检测金黄色葡萄球菌刺激后NETs的主要成分,DNA试剂盒提取测定游离DNA(f DNA)及酶联免疫吸附法测定(ELISA)上清LL-37,并分析其与SLE临床及实验指标的关系。结果 1)SLE稳定组、活动组、健康对照组3组金黄色葡萄球菌刺激后f DNA量的比较差异有统计学意义,SLE活动组、稳定组均高于健康对照组(P0.05),SLE活动组与稳定组无显著性差异(P0.05);LL-37比较无统计学意义(P0.05)。2)未经金黄色葡萄球菌刺激f DNA量3组间差异有统计学意义,SLE稳定组高于活动组和健康对照组(P分别是0.008、0.027),SLE活动组和健康对照组差异无统计学意义(P0.05);LL-37比较无统计学意义(P0.05)。3)3组体外金葡色葡萄球菌刺激后f DNA及LL-37量均较刺激前显著增加,差异均有统计学意义(f DNA P分别是0.000 1、0.000 1、0.001,LL-37 P均为0.000 1);刺激前后f DNA的差值SLE活动组显著高于稳定组和健康对照组(P0.05),SLE稳定组、健康对照组差异无统计学意义(P0.05);LL-37差值组间比较均无统计学意义(P0.05)。结论金黄色葡萄球菌刺激后,SLE患者NETs产生/释放量显著增加,SLE活动者最显著,金黄色葡萄球菌对SLE疾病活动可能有影响。
[Abstract]:Objective to investigate the effect of Staphylococcus aureus on the production of neutrophil net (NETs) in systemic lupus erythematosus (SLE). Methods Neutrophils were isolated and co-cultured with Staphylococcus aureus in 22 stable SLE patients, 20 active SLE patients and 18 healthy controls. The free DNA (f DNA) and enzyme linked immunosorbent assay (Elisa) supernatant LL-37 were extracted from NETs stimulated by Staphylococcus aureus and the relationship between them and the clinical and experimental indexes of SLE was analyzed. Results 1) there were significant differences in the f DNA content after staphylococcus aureus stimulation among stable SLE group, active SLE group and healthy control group. There was no significant difference between the active SLE group and the stable group (P0.05) there was no significant difference between the stable group and the active group (P0.05). There was no significant difference between the stable SLE group and the active group (P0.05). There was no significant difference between the stable SLE group and the active group without staphylococcus aureus stimulation (P0.05). There was no significant difference between active SLE group and healthy control group (P < 0.05). There was no significant difference between LL-37 group and healthy control group (P0.05). After stimulation of staphylococcus aureus in vitro, the levels of f DNA and LL-37 were significantly increased in three groups. The differences were statistically significant (f DNA P was 0.000 ~ 0.000 1g ~ (-1) L ~ (L-37) P = 0.000 1), the difference of f DNA in active SLE group was significantly higher than that in stable SLE group and healthy control group (P 0.05), and the difference of f DNA in active SLE group was significantly higher than that in stable SLE group before and after stimulation (P0.05). There was no significant difference between the healthy control group (P0.05) and the difference of LL-37 was not statistically significant (P0.05). Conclusion the NETs production / release in patients with SLE after staphylococcus aureus stimulation is the most significant in SLE patients. Staphylococcus aureus may have an effect on disease activity of SLE.
【作者单位】：安徽医科大学附属省立医院风湿免疫科;
【基金】：安徽省科技厅自然科学基金(1508085SMH229)
【分类号】：R593.241

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