GLP-1重组减毒沙门菌对2型糖尿病小鼠的血糖调控作用
发布时间：2018-09-18 17:47
【摘要】：目的观察重组减毒沙门菌作为胰高血糖素样肽-1(GLP-1)的基因载体,对2型糖尿病小鼠的血糖调控作用。方法高脂饲料饲喂4周后予以链脲佐菌素(STZ)腹腔注射构建KM种小鼠2型糖尿病模型,小鼠分为4组,每组10只,即:正常组、模型组、空载组及治疗组,分别予以:5%NaHCO_3、5%NaHCO_3、同等剂量的空载减毒沙门菌及GLP-1重组减毒沙门菌灌胃治疗,观察并记录治疗前后第0、7、14、21、28天的空腹血糖值及进食量、饮水量、体重变化,分别于治疗后第14天及第28天行口服葡萄糖耐量试验(OGTT)。取小鼠胰腺组织,采用HE染色法观察各组小鼠的胰腺组织形态学变化。结果 GLP-1重组减毒沙门菌灌胃治疗后,模型组小鼠空腹血糖水平较正常组升高,差异有统计学意义(P0.01),空载组空腹血糖与模型组比较,差异无统计学意义,治疗组空腹血糖与空载组比较降低,差异有统计学意义(P0.01),糖尿病小鼠体重变化有明显改善(P0.01),多饮多食症状好转;第14天及第28天OGTT示:空载组血糖与模型组比较,差异无统计学意义,治疗组血糖较空载组降低,差异有统计学意义(P0.01)。胰腺HE染色结果示,空载组与模型组比较,无明显变化,治疗组与空载组比较有明显改善。结论 GLP-1重组减毒沙门菌能够改善2型糖尿病小鼠多饮、多食、体重下降等症状,降低血糖,改善胰岛形态学变化,对2型糖尿病小鼠具有一定的治疗作用。
[Abstract]:Objective to observe the effect of recombinant attenuated Salmonella mutans on blood glucose in type 2 diabetic mice as a gene vector of glucagon like peptide 1 (GLP-1). Methods the type 2 diabetic model of KM mice was established by intraperitoneal injection of streptozotocin (STZ) in high fat diet for 4 weeks. The mice were divided into 4 groups with 10 mice in each group: normal group, model group, no-load group and treatment group. The rats were treated with 5% NaHCOS and 5 NaHCOs respectively. The same dose of non-loaded salmonella and GLP-1 recombinant attenuated salmonella were given intragastrically respectively. The fasting blood glucose value, the amount of food intake, the quantity of water consumed and the changes of body weight before and after treatment were observed and recorded. Oral glucose tolerance test (OGTT) (OGTT). Was administered on day 14 and day 28 after treatment, respectively. HE staining was used to observe the histomorphologic changes of the pancreas of the mice. Results after administration of GLP-1 recombinant attenuated Salmonella, the fasting blood glucose level in the model group was significantly higher than that in the normal group (P0.01), but there was no significant difference between the no-load group and the model group. The fasting blood glucose in the treatment group was significantly lower than that in the no-load group (P0.01), the weight change of diabetic mice was significantly improved (P0.01), and the symptoms of excessive drinking and eating were improved. OGTT on the 14th and 28th day showed that the blood glucose in the no-load group was higher than that in the model group. There was no significant difference in blood glucose in the treatment group compared with the no-load group, the difference was statistically significant (P0.01). The results of pancreatic HE staining showed that there was no significant change between the no-load group and the model group, but there was significant improvement in the treatment group and the no-load group. Conclusion GLP-1 recombinant attenuated Salmonella can improve the symptoms of type 2 diabetes, such as excessive drinking, eating and weight loss, decrease blood sugar, improve the morphological changes of pancreatic islets, and has a certain therapeutic effect on type 2 diabetic mice.
【作者单位】： 安徽医科大学第三附属医院内分泌科;上海交通大学Bio-x研究院上海交通大学实验动物中心;
【基金】：国家自然科学基金(编号:30840106)
【分类号】：R587.1
，
本文编号：2248659
[Abstract]:Objective to observe the effect of recombinant attenuated Salmonella mutans on blood glucose in type 2 diabetic mice as a gene vector of glucagon like peptide 1 (GLP-1). Methods the type 2 diabetic model of KM mice was established by intraperitoneal injection of streptozotocin (STZ) in high fat diet for 4 weeks. The mice were divided into 4 groups with 10 mice in each group: normal group, model group, no-load group and treatment group. The rats were treated with 5% NaHCOS and 5 NaHCOs respectively. The same dose of non-loaded salmonella and GLP-1 recombinant attenuated salmonella were given intragastrically respectively. The fasting blood glucose value, the amount of food intake, the quantity of water consumed and the changes of body weight before and after treatment were observed and recorded. Oral glucose tolerance test (OGTT) (OGTT). Was administered on day 14 and day 28 after treatment, respectively. HE staining was used to observe the histomorphologic changes of the pancreas of the mice. Results after administration of GLP-1 recombinant attenuated Salmonella, the fasting blood glucose level in the model group was significantly higher than that in the normal group (P0.01), but there was no significant difference between the no-load group and the model group. The fasting blood glucose in the treatment group was significantly lower than that in the no-load group (P0.01), the weight change of diabetic mice was significantly improved (P0.01), and the symptoms of excessive drinking and eating were improved. OGTT on the 14th and 28th day showed that the blood glucose in the no-load group was higher than that in the model group. There was no significant difference in blood glucose in the treatment group compared with the no-load group, the difference was statistically significant (P0.01). The results of pancreatic HE staining showed that there was no significant change between the no-load group and the model group, but there was significant improvement in the treatment group and the no-load group. Conclusion GLP-1 recombinant attenuated Salmonella can improve the symptoms of type 2 diabetes, such as excessive drinking, eating and weight loss, decrease blood sugar, improve the morphological changes of pancreatic islets, and has a certain therapeutic effect on type 2 diabetic mice.
【作者单位】： 安徽医科大学第三附属医院内分泌科;上海交通大学Bio-x研究院上海交通大学实验动物中心;
【基金】：国家自然科学基金(编号:30840106)
【分类号】：R587.1
，
本文编号：2248659
本文链接：https://www.wllwen.com/yixuelunwen/nfm/2248659.html
最近更新
教材专著
