人脐带间充质干细胞诱导分化不同阶段的胰岛样细胞移植治疗糖尿病

发布时间：2018-10-29 21:01

【摘要】：背景:实验室前期已对人脐带间充质干细胞进行不同时期的体外定向诱导,使其成功分化为胰岛样细胞,并检测到胰岛素表达。目的:观察人脐带间充质干细胞诱导分化不同阶段的胰岛样细胞在糖尿病大鼠模型体内的治疗效果,探索体外诱导的最佳时长。方法:将第3代人脐带间充质干细胞在体外定向诱导7,14,21,28 d使其分化为胰岛样细胞。选用清洁级健康Wistar大鼠80只,取8只做正常对照组,其余72只造模成功后,随机分为6组,分别为模型对照组、未诱导组、诱导7 d组、诱导14 d组、诱导21 d组、诱导28 d组。造模10 d后,分别经尾静脉移植相应的细胞悬液2 mL/只(细胞数量2×10~6个)。正常对照组和模型对照组经尾静脉注射不含任何细胞的培养液2 mL/只。每2周移植1次,共移植2次。治疗过程中检测各组大鼠的血糖、体质量、血清胰岛素水平,第2次细胞移植4周后处死大鼠,观察各脏器组织病理变化。结果与结论:①与模型对照组相比,各治疗组体质量上升,血糖下降,胰岛素水平升高,差异有显著性意义(P0.05),且诱导28 d组治疗效果最佳;②各治疗组病理切片显示,肝脏和肾脏组织结构清晰,未发生坏死、纤维化等异常改变;③实验结果表明,人脐带间充质干细胞诱导不同阶段的胰岛样细胞移植治疗糖尿病安全有效,体外诱导28 d的胰岛样细胞治疗效果最为显著。
[Abstract]:Background: human umbilical cord mesenchymal stem cells were induced in vitro to differentiate into islet like cells and insulin expression was detected. Aim: to observe the therapeutic effect of human umbilical cord mesenchymal stem cells (hCBSCs) on islet like cells in different stages of differentiation in diabetic rats. Methods: the third generation of human umbilical cord mesenchymal stem cells were induced to differentiate into islet like cells for 28 days. Eighty clean grade healthy Wistar rats were selected and 8 rats were used as normal control group. The other 72 rats were randomly divided into 6 groups: model control group, uninduced group, induced 7 d group, 14 d induction group and 21 d induction group. Induction 28 d group. After 10 days of modeling, the corresponding cell suspensions were transplanted through the tail vein for 2 mL/ (the number of cells was 2 脳 106). Normal control group and model control group were injected with culture medium without any cells for 2 mL/ via caudal vein. There were 2 transplants every 2 weeks. The blood glucose, body mass and serum insulin level were measured during the treatment. The rats were killed 4 weeks after the second cell transplantation, and the pathological changes of various organs were observed. Results and conclusion: 1Compared with the model control group, the body weight increased, the blood glucose decreased and the insulin level increased in each treatment group, the difference was significant (P0.05), and the treatment effect was the best in the 28d group. 2the pathological sections of each treatment group showed that the structure of liver and kidney was clear, no necrosis, fibrosis and other abnormal changes; 3 the results showed that islet like cell transplantation induced by human umbilical cord mesenchymal stem cells at different stages was safe and effective in the treatment of diabetes mellitus, and the effect of islet like cells induced 28 days in vitro was the most significant.
【作者单位】：北京大学航天临床医学院血液内分泌科;中国中医科学院中药研究所药理室;
【基金】：北京大学航天临床医学院科研基金资助项目(201302)~~
【分类号】：R587.1

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