VEGF在DN患者血清中的表达及其与微量白蛋白尿之间的相关性研究

发布时间：2018-12-27 14:48

【摘要】：目的探讨DN患者血清中VEGF表达水平的变化及其与尿微量白蛋白的关系,为DN蛋白尿的治疗提供新的治疗方向。方法54例糖尿病患者,依据24h尿微量白蛋白定量,将其分为三组:正常蛋白尿组、微量蛋白尿组、大量蛋白尿组,同期20例门诊健康体检者正常对照组。采用双抗体夹心酶联免疫吸附法(ELISE)检测血清中VEGF的水平,同时收集空腹血糖、血肌酐、尿素氮、糖化蛋白、收缩压及舒张压等临床指标。结果1.与对照组相比,微量及大量蛋白尿组血清中VEGF水平明显升高(P0.05),而正常蛋白尿组仅表现出上升趋势(P=0.630.05);2.与正常蛋白尿组比较,大量蛋白尿组血清中VEGF的表达水平明显升高(P0.05),而微量蛋白尿组则表现为上升趋势(P=0.0770.05);3.血清中VEGF的水平与空腹血糖(r=0.629,P0.01)、尿微量白蛋白(r=0.377,P0.01)呈显著正相关关系,与病程、血肌酐、尿素氮、尿酸、糖化蛋白、C-反应蛋白、收缩压及舒张压无相关关系。结论糖尿病肾病患者血清中VEGF的表达异常升高,并且与尿微量白蛋白呈正相关关系;抑制VEGF的过度表达,可能是一种防治蛋白尿形成的重要手段。
[Abstract]:Objective to investigate the changes of serum VEGF expression in patients with DN and its relationship with urinary microalbumin in order to provide a new therapeutic direction for the treatment of DN proteinuria. Methods 54 patients with diabetes mellitus were divided into three groups according to 24 hours urinary microalbuminuria: normal albuminuria group, microalbuminuria group, macroalbuminuria group, and normal control group of 20 healthy outpatients at the same time. Double antibody sandwich enzyme linked immunosorbent assay (ELISE) was used to detect the level of serum VEGF. Fasting blood glucose, serum creatinine, urea nitrogen, glycosylated protein, systolic blood pressure and diastolic blood pressure were collected. Result 1. Compared with the control group, the serum VEGF level in the microalbuminuria group and the massive proteinuria group was significantly higher (P0.05), while the normal proteinuria group only showed an upward trend (P0. 630. 05); 2. Compared with normal proteinuria group, the expression of VEGF in serum of large proteinuria group was significantly higher than that of microalbuminuria group (P0.05), while that of microalbuminuria group was increasing (P0. 077. 05); 3. The level of VEGF in serum was positively correlated with fasting blood glucose (r = 0.629, P 0.01) and urinary microalbumin (r = 0.377N, P 0.01), and with the course of disease, serum creatinine, urea nitrogen, uric acid, glycosylated protein, C-reactive protein, serum creatinine, urea nitrogen, uric acid, glycosylated protein, C-reactive protein. There was no correlation between systolic blood pressure and diastolic blood pressure. Conclusion the expression of VEGF in serum of patients with diabetic nephropathy is abnormally elevated and positively correlated with urinary albumin, and inhibiting the overexpression of VEGF may be an important means to prevent the formation of proteinuria.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R587.2;R692.9

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