骨髓间充质干细胞移植对去卵巢骨质疏松大鼠骨密度的影响

发布时间：2019-02-13 03:52

【摘要】：背景:骨髓间质干细胞可向成骨细胞分化,移植后可向病变部位迁移等特性,使其在骨质疏松中的研究受到越来越多的关注和重视。目的:观察骨髓间质干细胞移植对去卵巢骨质疏松大鼠骨密度的影响及其可能的作用机制。方法:(1)采用全骨髓贴壁培养法,从幼年SD大鼠股骨和胫骨骨髓中分离培养骨髓间质干细胞;(2)将成年雌性大鼠30只随机分为3组:假手术组、卵巢切除组、卵巢切除+骨髓间质干细胞移植组,每组10只。待骨质疏松模型建立成功后,卵巢切除+骨髓间质干细胞移植组大鼠经尾静脉注射含3.5×10~9 L~(-1)骨髓间质干细胞的PBS溶液(80μL/kg),其他2组注射等量的PBS溶液;(3)细胞移植第2周,采用双能X射线骨密度仪测量各组大鼠股骨骨密度、腰椎骨密度及全身骨密度,并测定血清钙、磷、碱性磷酸酶及骨钙素水平,micro-CT扫描进行胫骨组织微结构形态计量学分析。结果与结论:(1)卵巢切除组大鼠股骨骨密度、腰椎骨密度及全身骨密度显著低于假手术组;卵巢切除组大鼠血清中钙、骨钙素水平也显著低于假手术组,而磷、碱性磷酸酶水平明显升高,差异有显著性意义(P0.05或P0.01),以上表明骨质疏松大鼠模型成功制备;(2)与卵巢切除组大鼠相比,卵巢切除+骨髓间质干细胞移植组大鼠股骨、腰椎及全身骨密度均明显增加,血清中钙、骨钙素水平亦显著升高,而血清磷、碱性磷酸酶水平下降,差异均有显著性意义(P0.05);(3)与卵巢切除组比较,卵巢切除+骨髓间质干细胞移植组大鼠的骨体积分数、骨小梁数量和骨小梁厚度均显著增加,差异有显著性意义(P0.05);(4)结果表明,骨髓间质干细胞移植能够提高卵巢切除骨质疏松大鼠骨密度,促进骨形成。
[Abstract]:Background: bone marrow mesenchymal stem cells (BMSCs) can differentiate into osteoblasts and migrate to lesions after transplantation. Aim: to investigate the effect of bone marrow mesenchymal stem cell transplantation on bone mineral density in ovariectomized rats. Methods: (1) Bone marrow mesenchymal stem cells were isolated from femur and tibia bone marrow of young SD rats by whole bone marrow adherent culture. (2) Thirty adult female rats were randomly divided into three groups: sham-operated group and ovariectomized bone marrow mesenchymal stem cell transplantation group with 10 rats in each group. After the establishment of osteoporosis model, rats in ovariectomized bone marrow mesenchymal stem cell transplantation group were injected with PBS solution (80 渭 L/kg) containing 3.5 脳 10 9 L ~ (-1) bone marrow mesenchymal stem cells via caudal vein, and the other two groups were injected with the same amount of PBS solution. (3) at the 2nd week of cell transplantation, bone mineral density (BMD) of femur, lumbar vertebrae and whole body were measured by dual energy X-ray absorptiometry, and serum calcium, phosphorus, alkaline phosphatase and osteocalcin were measured. The microstructure of tibia was analyzed by micro-CT scanning. Results and conclusion: (1) the bone mineral density (BMD) of femur, lumbar vertebrae and whole body of ovariectomized rats were significantly lower than those of sham-operated group. The serum calcium and osteocalcin levels in ovariectomized rats were significantly lower than those in sham-operated rats, while the levels of phosphorus and alkaline phosphatase were significantly increased (P0.05 or P0.01). (2) compared with ovariectomized rats, bone mineral density of femur, lumbar vertebrae and whole body of ovariectomized bone marrow mesenchymal stem cell transplantation rats were significantly increased, serum calcium and osteocalcin levels were also significantly increased, and serum phosphorus levels were also significantly increased. The level of alkaline phosphatase decreased significantly (P0.05). (3) compared with the ovariectomized group, the bone volume fraction, the number of trabecular bone and the thickness of trabecular bone in ovariectomized bone marrow mesenchymal stem cell transplantation group were significantly increased (P0.05); (4) the results showed that bone marrow mesenchymal stem cell transplantation could increase bone mineral density and promote bone formation in ovariectomized osteoporosis rats.
【作者单位】：广东医学院附属医院骨外科;
【基金】：广东省湛江市2015科技计划项目(2015A01018)~~
【分类号】：R580

【参考文献】