雌激素与尾悬吊法导致雄性C57小鼠骨质疏松关系的研究

发布时间：2019-04-20 16:17

【摘要】：目的探讨雌激素对尾悬吊导致雄性C57小鼠骨质疏松的影响及其内在机制。方法 32只成年C57BL/6J雄性小鼠,随机分成4组:对照组(control,C),模型组(model,M),雌激素组(estrogen,E),雌激素+雌激素受体抑制组(estrogen+ICI 182,780,E+I)。除C组外其余组小鼠尾悬吊两周,所有小鼠在第15d处死,取小鼠血清进行生化指标分析,取小鼠右侧股骨进行显微CT扫描和形态学染色,取小鼠左侧股骨进行生物力学检测并取股骨远端研磨后进行蛋白检测。结果尾悬吊两周导致雄性C57小鼠股骨出现明显骨质疏松,M组与C组相比差异有显著的统计学意义(P0.05)。雌激素干预能抑制骨质流失,E组多数骨相关参数与对照组相比,差异无统计学意义(P0.05)。而使用雌激素受体拮抗剂ICI182,780之后,雌激素骨保护效应消失,与M组相比差异无统计学意义(P0.05)。尾悬吊两周导致股骨远端离子型谷氨酸受体NMDA亚型NR2A表达降低,雌激素升高骨组织中NR2A表达水平。结论雄性C57小鼠尾悬吊2w表现出显著骨质疏松,雌激素通过激活雌激素受体明显改善骨质流失,可能是通过促进骨组织中NR2A表达而实现成骨细胞增殖和分化。
[Abstract]:Objective to investigate the effect of estrogen on osteoporosis induced by tail suspension in male C57 mice and its underlying mechanism. Methods 32 adult C57BL/6J male mice were randomly divided into 4 groups: control group (control,C), model group (model,M), estrogen group (estrogen,E), estrogen receptor inhibition group (estrogen ICI 182, 780, E I). The mice in the other groups were suspended for two weeks except group C, all the mice were killed on the 15th day, the serum of the mice was taken for biochemical index analysis, and the right femur of the mice was taken for microscopic CT scanning and morphological staining. The left femur of the mice was taken for biomechanical examination and the distal femur was ground for protein detection. Results two weeks of tail suspension resulted in significant osteoporosis in the femur of male C57 mice, and there was significant difference between M group and C group (P0.05). Estrogen intervention can inhibit bone loss, E group compared with the control group, most of the bone-related parameters, there is no significant difference (P0.05). When estrogen receptor antagonist ICI182780 was used, the bone protective effect of estrogen disappeared, and there was no significant difference compared with M group (P0.05). Tail suspension for two weeks resulted in the decrease of NR2A expression of ionized glutamate receptor NMDA subtype in distal femur and the elevation of NR2A expression in bone tissue by estrogen. Conclusion male C57 mice showed significant osteoporosis after 2 weeks of tail suspension. Estrogen could significantly improve bone loss by activating estrogen receptor, which might be related to the proliferation and differentiation of osteoblasts by promoting the expression of NR2A in bone tissue.
【作者单位】：南京大学医学院附属金陵医院骨科;第四军医大学基础部药理学教研室;
【基金】：江苏省六大人才高峰资助(2013-WSN-091) 江苏省临床科技项目基金(BL2012002)
【分类号】：R580

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