雌二醇对去卵巢大鼠骨和生殖系统损伤的修复作用
发布时间：2019-06-07 17:19
【摘要】：妇女绝经后因卵巢功能下降、雌激素水平骤降,体内骨转换率显著加快,骨吸收的速度超过骨形成的速度,导致骨量丢失、骨密度下降、骨骼脆性增加引起的骨质疏松症称为绝经后骨质疏松症(postmenopausal osteoporosis,PMOP)。妇女绝经后雌激素分泌锐减,加速骨质的流失,PMOP俨然成为一种严重危害社会公共健康的高发性疾病,也是国际骨病研究中的热点和难点。本研究通过观察雌二醇对绝经后骨质疏松大鼠骨组织、子宫、阴道结构损伤的影响,以及雌二醇对骨质疏松大鼠骨组织肥大细胞增生的影响,探讨雌二醇对骨质疏松大鼠骨组织和生殖系统损伤的修复作用及肥大细胞作为绝经后骨质疏松症诊断和治疗依据的可能性。本研究以3月龄SD大鼠构建绝经后骨质疏松大鼠模型,然后通过腹腔注射雌二醇进行治疗性给药。给药结束后颈椎脱臼处死大鼠,计算各脏器指数,采用火焰原子吸收法检测血清钙(Ca)含量,ELISA法检测血清中的雌二醇(E2)和碱性磷酸酶(ALP)水平;股骨、胫骨、股骨头组织固定和脱钙后制作石蜡切片,进行HE染色和甲苯胺蓝染色,应用Image-Pro Plus图像分析系统进行形态计量学分析,显微计数骨组织肥大细胞数量;取子宫和阴道组织常规石蜡切片后HE染色,显微观察子宫、阴道的组织病理学变化,并进行形态计量学分析。研究结果表明:去卵巢组大鼠较假手术组血清Ca显著降低(P0.05)、血清E2显著降低(P0.01)、血清ALP显著升高(P0.01),子宫指数显著降低(P0.01);去卵巢组大鼠股骨干骺端和股骨头的骨小梁面积百分率和骨小梁厚度较假手术组显著降低(P0.01),骨小梁间距增大(P0.01);去卵巢组股骨和股骨头横断面全层的肥大细胞数量较假手术组显著增多(P0.01);子宫管径厚度降低(P0.01)、子宫黏膜上皮厚度和子宫腺体数量降低(P0.05);阴道黏膜上皮厚度和肌层血管数量降低(P0.01),固有层血管数量减少(P0.05),而给药雌二醇治疗后以上症状均得到缓解。由研究结果得到以下结论:3月龄SD大鼠去除卵巢后3个月可成功建立绝经后骨质疏松疾病模型;间歇性腹腔注射雌二醇可有效改善去卵巢骨质疏松大鼠的骨代谢生化指标以及骨组织微结构,具有防治绝经后骨质疏松症的作用;雌二醇可有效抑制去卵巢骨质疏松大鼠的骨肥大细胞增生,骨肥大细胞有望成为绝经后骨质疏松症诊断和治疗的依据;雌二醇可有效缓解去卵巢骨质疏松大鼠子宫和阴道的萎缩,对绝经后骨质疏松大鼠生殖系统损伤具有修复作用。
[Abstract]:In postmenopausal women, due to the decrease of ovarian function, the level of estrogen plummeted, the bone turnover rate in vivo was significantly accelerated, the rate of bone resorption exceeded the rate of bone formation, resulting in bone mass loss and decreased bone mineral density. Osteoporosis caused by increased bone fragility is called postmenopausal osteoporosis (postmenopausal osteoporosis,PMOP). With the sharp decrease of estrogen secretion and accelerated bone loss in postmenopausal women, PMOP has become a high incidence disease which seriously endangers social and public health, and is also a hot and difficult point in international bone disease research. The purpose of this study was to observe the effects of estrogen on bone tissue, uterus and vaginal structure injury in postmenopausal osteoporosis rats, and the effect of estrogen on mastocyte proliferation in bone tissue of osteoporotic rats. To investigate the repair effect of estrogen on bone tissue and reproductive system injury in osteoporotic rats and the possibility of mast cells as the basis for the diagnosis and treatment of postmenopausal osteoporosis. In this study, 3-month-old SD rats were used to establish the rat model of postmenopausal osteoporosis, and then Estradiol was injected intraperitoneally for therapeutic administration. After administration, the rats were killed after cervical dislocated. The indexes of each organ were calculated. The content of serum calcium (Ca) was measured by flame atomic absorption spectrometry, and the levels of estrogen (E2) and alkaline phosphatase (ALP) in serum were measured by ELISA method. Paraffin sections were made after fixation and decalcification of femur, tibia and femoral head. HE staining and toluidine blue staining were performed. Morphometric analysis was carried out by Image-Pro Plus image analysis system, and the number of mastocytes in bone tissue was counted microscopically. The pathological changes of uterus and vagina were observed by HE staining after routine paraffin sections of uterus and vagina, and morphometric analysis was carried out. The results showed that serum Ca, E2, ALP and uterine index in ovariectomy group were significantly lower than those in pseudo-operation group (P 0.05), P < 0.01, P 0.01, P < 0.01, respectively. The percentage of bone trabecular area and the thickness of bone cerebellum in ovariectomy group were significantly lower than those in false operation group (P 0.01), and the distance between bone trabeculae was increased (P 0.01). Compared with the pseudo-operation group, the number of mastocytes in the cross section of the femur and femoral head in the ovariectomy group was significantly increased (P 0.01), the thickness of uterine canal diameter was decreased (P 0.01), the thickness of uterine mucous membrane and the number of uterine gland were decreased (P 0.05), and the thickness of uterine canal diameter was decreased (P < 0.01). The thickness of vaginal mucosa and the number of vessels in muscle layer decreased (P 0.01), the number of vessels in lamina propria decreased (P 0.05), and the above symptoms were relieved after treatment with estridiol. The conclusions are as follows: 3 months old SD rats can successfully establish postmenopausal osteoporosis model 3 months after ovariectomy. Intermittent intraperitoneal injection of estrogen can effectively improve the biochemical indexes of bone metabolism and bone tissue microstructure in ovariectomy osteoporosis rats, and has the effect of preventing and treating postmenopausal osteoporosis. Estradiol can effectively inhibit the proliferation of bone hypertrophic cells in ovariectomy osteoporosis rats, and bone hypertrophic cells are expected to be the basis for the diagnosis and treatment of postmenopausal osteoporosis. Estradiol can effectively alleviate the atrophy of uterus and vagina in ovariectomy osteoporosis rats and repair the reproductive system injury in postmenopausal osteoporosis rats.
【学位授予单位】：陕西理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R580
本文编号：2494961
[Abstract]:In postmenopausal women, due to the decrease of ovarian function, the level of estrogen plummeted, the bone turnover rate in vivo was significantly accelerated, the rate of bone resorption exceeded the rate of bone formation, resulting in bone mass loss and decreased bone mineral density. Osteoporosis caused by increased bone fragility is called postmenopausal osteoporosis (postmenopausal osteoporosis,PMOP). With the sharp decrease of estrogen secretion and accelerated bone loss in postmenopausal women, PMOP has become a high incidence disease which seriously endangers social and public health, and is also a hot and difficult point in international bone disease research. The purpose of this study was to observe the effects of estrogen on bone tissue, uterus and vaginal structure injury in postmenopausal osteoporosis rats, and the effect of estrogen on mastocyte proliferation in bone tissue of osteoporotic rats. To investigate the repair effect of estrogen on bone tissue and reproductive system injury in osteoporotic rats and the possibility of mast cells as the basis for the diagnosis and treatment of postmenopausal osteoporosis. In this study, 3-month-old SD rats were used to establish the rat model of postmenopausal osteoporosis, and then Estradiol was injected intraperitoneally for therapeutic administration. After administration, the rats were killed after cervical dislocated. The indexes of each organ were calculated. The content of serum calcium (Ca) was measured by flame atomic absorption spectrometry, and the levels of estrogen (E2) and alkaline phosphatase (ALP) in serum were measured by ELISA method. Paraffin sections were made after fixation and decalcification of femur, tibia and femoral head. HE staining and toluidine blue staining were performed. Morphometric analysis was carried out by Image-Pro Plus image analysis system, and the number of mastocytes in bone tissue was counted microscopically. The pathological changes of uterus and vagina were observed by HE staining after routine paraffin sections of uterus and vagina, and morphometric analysis was carried out. The results showed that serum Ca, E2, ALP and uterine index in ovariectomy group were significantly lower than those in pseudo-operation group (P 0.05), P < 0.01, P 0.01, P < 0.01, respectively. The percentage of bone trabecular area and the thickness of bone cerebellum in ovariectomy group were significantly lower than those in false operation group (P 0.01), and the distance between bone trabeculae was increased (P 0.01). Compared with the pseudo-operation group, the number of mastocytes in the cross section of the femur and femoral head in the ovariectomy group was significantly increased (P 0.01), the thickness of uterine canal diameter was decreased (P 0.01), the thickness of uterine mucous membrane and the number of uterine gland were decreased (P 0.05), and the thickness of uterine canal diameter was decreased (P < 0.01). The thickness of vaginal mucosa and the number of vessels in muscle layer decreased (P 0.01), the number of vessels in lamina propria decreased (P 0.05), and the above symptoms were relieved after treatment with estridiol. The conclusions are as follows: 3 months old SD rats can successfully establish postmenopausal osteoporosis model 3 months after ovariectomy. Intermittent intraperitoneal injection of estrogen can effectively improve the biochemical indexes of bone metabolism and bone tissue microstructure in ovariectomy osteoporosis rats, and has the effect of preventing and treating postmenopausal osteoporosis. Estradiol can effectively inhibit the proliferation of bone hypertrophic cells in ovariectomy osteoporosis rats, and bone hypertrophic cells are expected to be the basis for the diagnosis and treatment of postmenopausal osteoporosis. Estradiol can effectively alleviate the atrophy of uterus and vagina in ovariectomy osteoporosis rats and repair the reproductive system injury in postmenopausal osteoporosis rats.
【学位授予单位】：陕西理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R580
【参考文献】
相关期刊论文 前10条
1 辛薇;祁珊珊;曾海涛;;雌激素对去卵巢骨质疏松大鼠子宫组织形态的影响[J];黑龙江畜牧兽医;2016年15期
2 郑红星;祁珊珊;曾海涛;;葛根异黄酮联合VitD_3对去卵巢骨质疏松大鼠骨组织构造的影响[J];中国骨质疏松杂志;2016年07期
3 赵雨坤;郑康;郭晴晴;樊丹平;何小鹃;吕爱平;;一种基于EDTA脱钙法的改良骨组织脱钙方法[J];中国实验方剂学杂志;2016年11期
4 祁珊珊;王永吉;辛薇;;SD大鼠绝经后骨质疏松疾病动物模型的构建[J];临床与实验病理学杂志;2016年01期
5 祁珊珊;王永吉;庞田田;杨yN琦;吴婕;辛薇;;去卵巢大鼠骨、生殖系统组织病理学与形态计量学观察[J];中国骨质疏松杂志;2015年07期
6 姚国荣;;雌二醇和孕酮对双侧卵巢切除小鼠子宫局部巨噬细胞及M-CSF表达的影响研究[J];中国现代医生;2015年18期
7 祁珊珊;杨yN琦;庞田田;王永吉;;去卵巢法建立SD大鼠绝经后骨质疏松模型手术探讨[J];中国兽医杂志;2014年02期
8 邝志强;张荣华;;运动联合阿仑膦酸钠片对去卵巢大鼠RANKL/OPG平衡及MAPK信号通路的影响[J];第二军医大学学报;2013年08期
9 孙东霞;;绝经后骨质疏松症患者综合治疗前后生活质量分析[J];内蒙古医学杂志;2011年S8期
10 郑家三;夏成;朱玉哲;张洪友;徐闯;;日粮能量水平对奶牛繁殖性能、血浆代谢产物和生殖激素的影响[J];中国奶牛;2011年10期
相关博士学位论文 前1条
1 耿文鑫;抗绝经后骨质疏松蛋白疫苗的实验研究[D];西北大学;2013年
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